IL20RA elisa pair set
IL20RA Matched ELISA Antibody Pair Set, Human
Synonyms
IL20RA; IL20RA Matched ELISA Antibody Pair Set; Human; Human IL20RA/IL20R1 ELISA Pair Set; interleukin 20 receptor; alpha; CRF2-8 Matched ELISA Antibody Pair Set; IL-20R-alpha Matched ELISA Antibody Pair Set; IL-20R1 Matched ELISA Antibody Pair Set; IL-20RA Matched ELISA Antibody Pair Set; IL20RA elisa pair set
Specificity
Quantitative determination of Human IL20RA
Assay Type
Solid Phase Sandwich ELISA
Detection Range
31.25-2000pg/mL
Sensitivity
31.25pg/ml
Materials Provided
1. Capture Antibody 0.5 mg/mL of mouse anti-IL20RA monoclonal antibody. Dilute to a working concentration of 1ug/mL in CBS before coating.
2. Detection Antibody 0.5 mg/mL rabbit anti-IL20RA polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 70 ng of recombinant IL20RA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2ng/mL is recommended.
2. Detection Antibody 0.5 mg/mL rabbit anti-IL20RA polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 70 ng of recombinant IL20RA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2ng/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Related Product Information for IL20RA elisa pair set
Interleukin 20 receptor, alpha subunit (IL20RA/IL-20RA) also known as IL-20 receptor subunit alpha, Cytokine receptor class-II member 8, interleukin-20 receptor I, and interleukin-20 receptor subunit alpha, is a subunit for the interleukin-20 receptor. IL20RA/IL-20RA belongs to the type II cytokine receptor family. This cytokine is a receptor for interleukin 20 (IL20), a cytokine that may be involved in the epidermal function. The receptor of IL20 is a heterodimeric receptor complex consisting of this protein and interleukin 20 receptor beta (IL20B). IL20RA forms heterodimer with IL20RB and the complex serves as a receptor for IL19, IL20, and IL24. All three are capable of signaling through the IL-20RA/IL-20RB complex. The ligand binding to receptor B creating a high-affinity binding site for receptor A which is recruited to complete the complex. Besides, IL20RA also forms a heterodimer with the unique and specific receptor IL10RB and functions as the receptor for IL26. IL20RA is widely expressed with the highest levels in skin, testis, and brain. The expression of both IL20RA and IL20RB is found to be upregulated in psoriatic skin lesions on keratinocytes.
This IL20RA Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human IL20RA. It contains Human IL20RA capture antibody, Human IL20RA detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human IL20RA protein.
This IL20RA Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human IL20RA. It contains Human IL20RA capture antibody, Human IL20RA detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human IL20RA protein.
References
Parrish-Novak J, et al. (2002) Interleukins 19, 20, and 24 signal through two distinct receptor complexes. Differences in receptor-ligand interactions mediate unique biological functions. J Biol Chem. 277(49): 47517-23.Kingo K, et al. (2008) Association analysis of IL20RA and IL20RB genes in psoriasis. Genes Immun. 9(5): 445-51.Blumberg H, et al. (2001) Interleukin 20: discovery, receptor identification, and role in epidermal function. Cell. 104(1): 9-19.
