Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human small intestine tissue using MBS7004144 at dilution of 1:100)
Rabbit anti-Human MOB kinase activator 1A Polyclonal Antibody | anti-MOB1A antibody
Rabbit anti-human MOB kinase activator 1A polyclonal Antibody
IHC: 1:100-1:1000
IF: 1:200-1:500
IP: 1:200-1:2000
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human small intestine tissue using MBS7004144 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human small intestine tissue using MBS7004144 at dilution of 1:100)
Western Blot (WB)
(Western BlotPositive WB detected in: K562 whole cell lysate, Jurkat whole cell lysateAll lanes: MOB1A antibody at 3.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 26, 17 kDaObserved band size: 26 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: K562 whole cell lysate, Jurkat whole cell lysateAll lanes: MOB1A antibody at 3.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 26, 17 kDaObserved band size: 26 kDa)
Immunohistochemistry (IHC)
(IHC image of MBS7004144 diluted at 1:1000 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of MBS7004144 diluted at 1:1000 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunofluorescence (IF)
(Immunofluorescence staining of HepG2 cells with MBS7004144 at 1:333, counter- stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of HepG2 cells with MBS7004144 at 1:333, counter- stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Immunoprecipitating (IP)
(Immunoprecipitating MOB1A in Jurkat whole cell lysateLane 1: Rabbit control IgG instead of MBS7004144 in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7004144 (5ug) + Jurkat whole cell lysate (500ug)Lane 3: Jurkat whole cell lysate (20ug))
Immunoprecipitating (IP)
(Immunoprecipitating MOB1A in Jurkat whole cell lysateLane 1: Rabbit control IgG instead of MBS7004144 in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7004144 (5ug) + Jurkat whole cell lysate (500ug)Lane 3: Jurkat whole cell lysate (20ug))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a component of the Hippo signaling pathway, which controls organ size and tumor growth by enhancing apoptosis. Loss of the encoded protein results in cell proliferation and cancer formation. The encoded protein is also involved in the control of microtubule stability during cytokinesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
Uniprot Description
MOB1A: Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L. Acts cooperatively with STK3/MST2 to activate STK38. Binds STK38 and STK38L. Interacts with LATS1 and LATS2. Forms a tripartite complex with STK38 and STK3/MST2. Adrenal gland, bone marrow, brain, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, heart, spinal cord, fetal brain and fetal liver. Belongs to the MOB1/phocein family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, regulatory subunit
Chromosomal Location of Human Ortholog: 2p13.1
Cellular Component: cytosol
Molecular Function: metal ion binding; protein binding
Research Articles on MOB1A
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Product Notes
The MOB1A mob1a (Catalog #AAA7004144) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human MOB kinase activator 1A polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MOB kinase activator 1A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:2000-1:5000 IHC: 1:100-1:1000 IF: 1:200-1:500 IP: 1:200-1:2000. Researchers should empirically determine the suitability of the MOB1A mob1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MOB kinase activator 1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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