Urokinase/uPA ELISA Pair Set | PLAU elisa pair set

Urokinase/uPA Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118305

• Synonyms: Urokinase/uPA; Urokinase/uPA Matched ELISA Antibody Pair Set; Human; Human Urokinase/PLAU/UPA ELISA Pair Set; plasminogen activator; urokinase; ATF Matched ELISA Antibody Pair Set; BDPLT5 Matched ELISA Antibody Pair Set; QPD Matched ELISA Antibody Pair Set; u-PA Matched ELISA Antibody Pair Set; UPA Matched ELISA Antibody Pair Set; URK Matched ELISA Antibody Pair Set; PLAU elisa pair set

• Specificity: Quantitative determination of Human Urokinase/uPA

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 4.69-300pg/mL
• Sensitivity: 4.69pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of mouse anti-Human Urokinase/PLAU/UPA monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of mouse anti-Human Urokinase/PLAU/UPA monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 15 ng of recombinant Human Urokinase/PLAU/UPA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 300 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for PLAU elisa pair set

Plasminogen activator, urokinase, also known as PLAU and uPA, is a serine protease which converts plasminogen to plasmin, a broad-spectrum protease active on extracellular matrix (ECM) components. It is involved in complement activation, cell migration, wound healing, and generation of localized extracellular proteolysis during tissue remodelling, pro-hormone conversion, carcinogenesis and neoplasia. Like many components of the blood coagulation, fibrinolytic and complement cascades, uPA has a modular structure, including three conserved domains: a growth factor-like domain (GFD, residues 1-49), a kringle domain (residues 50-131), linked by an interdomain linker or "connecting peptide" (CP, residues 132-158) to the serine protease domain (residues 159-411). uPA and its receptor (uPAR) have been implicated in a broad spectrum of pathophysiological processes, including fibrinolysis, proteolysis, inflammation, atherogenesis and plaque destabilization, all of which are involved in the pathogenesis of MI (myocardial infarction). The role of uPA is not only linked to its action as an enzyme. In fact, the mere binding of uPA on the cell surface also brings about two events that broaden the spectrum of its biological functions: (1) a conformational change of the receptor, which, in turn, affects its interaction with other proteins; (2) a signal transduction which modulates the expression of apoptosis-related genes. Besides its applications as a thrombolytic agent and as a prognostic marker for tumors, uPA may provide the basis for other therapies, as the structure of the receptor-binding domain of uPA has become a model for the design of anti-cancer molecules. Because of the causal involvment of uPA in cancer invasion and metastasis, the blockade of uPA interactions and activity with specific inhibitors is of interest for novel strategies in cancer therapy.

This Urokinase/uPA Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Urokinase/uPA. It contains Human Urokinase/uPA capture antibody, Human Urokinase/uPA detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Urokinase/uPA protein.

References

Crippa MP. (2007) Urokinase-type plasminogen activator. Int J Biochem Cell Biol. 39(4): 690-4.Kunamneni A, et al. (2008) Urokinase-a very popular cardiovascular agent. Recent Pat Cardiovasc Drug Discov. 3(1): 45-58.Vincenza Carriero M, et al. (2009) Structure, function and antagonists of urokinase-type plasminogen activator. Front Biosci. 14: 3782-94.Xu J, et al. (2010) Association of putative functional variants in the PLAU gene and the PLAUR gene with myocardial infarction. Clin Sci (Lond). 119(8): 353-9.