Western Blot (WB)
(Western blot analysis of RAD50 expression in HeLa (A); K562 (B); Jurkat (C); MCF7 (D); Ramos (E); SW480 (F) whole cell lysates.)
Rabbit RAD50 Polyclonal Antibody | anti-RAD50 antibody
RAD50 Antibody
Gene Names
RAD50; NBSLD; RAD502; hRad50
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Immunogen Affinity Chromatography
Synonyms
RAD50; Polyclonal Antibody; RAD50 Antibody; Purified Polyclonal RAD50 Antibody; Polyclonal RAD50 antibody; Anti-RAD50 antibody; DNA repair protein RAD50 antibody; hRAD50 antibody; anti-RAD50 antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of RAD50 protein
Purity/Purification
Immunogen Affinity Chromatography
Form/Format
Supplied in liquid form in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3 with 30% Glycerol and 0.01% Sodium Azide
Sequence Length
1312
Applicable Applications for anti-RAD50 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IHC: 1:100-1:200
IP: 1:10-1:100
IHC: 1:100-1:200
IP: 1:10-1:100
Immunogen
RAD50 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RAD50 as the immunogen
Cross-Reactivity
Mouse, Rat, Chicken
Preparation and Storage
Store at 4 degree C for short term storage. For long term, aliquot and store at -20 degree C. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of RAD50 expression in HeLa (A); K562 (B); Jurkat (C); MCF7 (D); Ramos (E); SW480 (F) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of RAD50 expression in HeLa (A); K562 (B); Jurkat (C); MCF7 (D); Ramos (E); SW480 (F) whole cell lysates.)
Related Product Information for anti-RAD50 antibody
RAD50 is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1 (also known as Xrs2 in yeast). This MRN complex (MRX complex in yeast) binds to broken DNA ends and displays numerous enzymatic activities that are required for double-strand break repair by nonhomologous end-joining or homologous recombination.
Product Categories/Family for anti-RAD50 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Rad50
NCBI Official Synonym Full Names
RAD50 double strand break repair protein
NCBI Official Symbol
RAD50
NCBI Official Synonym Symbols
NBSLD; RAD502; hRad50
NCBI Protein Information
DNA repair protein RAD50
Protein Family
NCBI Description
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]
Research Articles on RAD50
Similar Products
Product Notes
The RAD50 (Catalog #AAA5315392) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RAD50 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC: 1:100-1:200 IP: 1:10-1:100. Researchers should empirically determine the suitability of the RAD50 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAD50, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
