RBP4 elisa kit

RBP4 (mouse / rat) ELISA Kit

Cat. Num. AAA843367

• Gene Names: RBP4; RDCCAS; MCOPCB10
• Synonyms: RBP4; RBP4 (mouse / rat) ELISA Kit; RBP4 elisa kit

• Sequence Length: 201

• Samples: Serum, urine, or Cell Culture Supernatant
• Assay Type: Sandwich
• Features & Benefits: Simple procedure; Fast and convenient; High-throughput adaptable
• Preparation and Storage: 4 degree C; Shelf Life: 12 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RBP4 elisa kit

Product Information: RBP4 (mouse/rat) ELISA Kit: Colorimetric Assay for in vitro Quantitative Determination of Mouse or Rat RBP4 in Serum, Plasma, Urine and Cell Culture Supernatant. Assay Range 0.188 - 12 ng RBP4/ml and Detection limit 60 pg/ml. 100 Assays.

Background: Retinol binding protein (RBP) 4 is the only specific transport protein for vitamin A in the circulation whose function is to deliver vitamin to target tissues. In obesity and type 2 diabetes, expression of Glut4 is significantly impaired in adipocytes. Glucose transport via Glut4 is the rate-limiting step for glucose use by muscle and adipose tissue. Adipocyte-specific deletion of Gluts leads to notable elevation of mouse RBP4 causing systemic insulin resistance, and that reduction of RBP4 improves insulin resistance. This identified a novel role of RBP4 in regulating insulin action and RBP4 is recorded as an adipocyte-derived hormone. The RBP4 (mouse/rat) ELISA Kit is to be used for the in vitro quantitative determination of mouse or rat RBP4 in serum, urine and cell culture supernatant. This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG, peroxidase and TMB (3,3',5,5'-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of RBP4 in the samples. This ELISA is specific for the measurement of natural and recombinant mouse and rat RBP4. It does not cross-react with human RBP4, mouse adiponectin, mouse resistin, mouse Nampt, rat adiponectin, rat resistin, rat Nampt, mouse RELM-alpha, mouse RELM-beta, human leptin, rat RELM-alpha, mouse 41BBL, mouse CD137, mouse Tim3, human TNF-alpha. The assay range is 0.188 - 12 ng RBP4/ml and a detection limit of 60 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).

Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Applications- This assay detects mouse/rat RBP4 in the range of 0.188 - 12 ng RBP4/ml and a detection limit of 60 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).

Product Categories/Family for RBP4 elisa kit

Diabetes; Obesity & Metabolic Syndrome; Lipid Metabolism; Assay Kits; Diabetes; Obesity & Metabolic Syndrome; Signaling Pathways in Diabetes and Obesity; Adipokine Signaling; Assay Kits; Metabolism Assays; Lipids MetabolismMetabolism Assays; Metabolism As

NCBI and Uniprot Product Information

• NCBI GI #: 55743122
• NCBI GeneID: 5950
• NCBI Accession #: NP_006735.2
• NCBI GenBank Nucleotide #: NM_006744.3
• Molecular Weight: 23,010 Da
• NCBI Official Full Name: retinol-binding protein 4
• NCBI Official Synonym Full Names: retinol binding protein 4, plasma
• NCBI Official Symbol: RBP4
• NCBI Official Synonym Symbols: RDCCAS; MCOPCB10
• NCBI Protein Information: retinol-binding protein 4
• UniProt Protein Name: Retinol-binding protein 4
• Protein Family: Retinol-binding protein
• UniProt Gene Name: RBP4
• UniProt Synonym Gene Names: PRBP; RBP
• UniProt Entry Name: RET4_HUMAN

NCBI Description

This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008]

Uniprot Description

RBP4: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency). This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q23.33

Cellular Component: extracellular space; protein complex; extracellular region; cytosol

Molecular Function: protein binding; protein heterodimerization activity; retinol binding; retinal binding

Biological Process: phototransduction, visible light; retinal metabolic process; embryonic skeletal development; maintenance of gastrointestinal epithelium; heart development; uterus development; glucose homeostasis; response to insulin stimulus; embryonic organ morphogenesis; negative regulation of cardiac muscle cell proliferation; retinol metabolic process; detection of light stimulus involved in visual perception; retinoid metabolic process; female genitalia morphogenesis; cardiac muscle development; response to retinoic acid; urinary bladder development; male gonad development; vagina development; positive regulation of insulin secretion; positive regulation of immunoglobulin secretion; gluconeogenesis; response to ethanol; eye development; embryonic retina morphogenesis in camera-type eye; spermatogenesis; lung development

Disease: Retinal Dystrophy, Iris Coloboma, And Comedogenic Acne Syndrome; Microphthalmia, Isolated, With Coloboma 10