Typical Testing Data/Standard Curve (for reference only)
Homocysteine ELISA Kit
Homocysteine ELISA Kit
Synonyms
Homocysteine; Homocysteine ELISA Kit; Homocysteine elisa kit
Preparation and Storage
Upon receipt, store Homocysteine Conjugate (1000X) and Homocysteine-BSA Standard at -20 degree C
Store the rest of the kit at 4 degree C.
Store the rest of the kit at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Homocysteine elisa kit
Background/Introduction: Hexosaminidase is an enzyme that hydrolyzes terminal N-acetyl-D-hexosamine residues from N-acetyl-beta-D-hexosaminides. The enzyme, localized to cellular lysosomes, is a dimer formed from either an alpha or beta subunit which produces three possible dimer combinations. The alphabeta combination (isozyme A) is the only combination with a known function: hydrolyzing GM2 ganglioside in vivo. The other two combinations of alphaalpha (isozyme B) and betabeta (isozyme S) have been observed in cellular tissues but their function is unknown. Isozyme A works in combination with cofactor GM2 activator protein to degrade GM2 gangliosides and other molecules that have terminal N-acetyl hexosamines. Gene mutations that code for the beta subunit can result in Sandhoff disease. Mutations in the locus for the alpha subunit decrease the hydrolysis of GM2 gangliosides, which is the primary cause of Tay-Sachs disease. While both the alpha and beta subunits of lysosomal hexosaminidase can cleave GalNAc residues, only the alpha subunit is able to hydrolyze GM2 gangliosides because of a key residue, Arg-424, and a loop structure that forms from the amino acid sequence in the alpha subunit. The GM2 activator protein acts as a transporter to localize GM2 gangliosides to hexosaminidase, so a functional hexosaminidase enzyme is able to hydrolyze GM2 gangliosides into GM3 gangliosides. The Beta Hexosamindase Activity Assay Kit is a simple fluorometric assay that measures beta hexosaminidase activity in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, beta hexosaminidase positive control and unknown samples.
Product Categories/Family for Homocysteine elisa kit
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Product Notes
The Homocysteine (Catalog #AAA169254) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Homocysteine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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