C. Perfringens Neuraminidase Active Protein | NA active protein

C. Perfringens Neuraminidase Protein, C.perfringens, Recombinant (His Tag)

Cat. Num. AAA8120911

• Purity: >97% as determined by SDS-PAGE
• Synonyms: C. Perfringens Neuraminidase; C. Perfringens Neuraminidase Protein; C.perfringens; Recombinant (His Tag); C. Perfringens Neuraminidase/NA (Active)(His Tag); NA Protein; NA active protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Cys2-Gln382

• Species: C. Perfringens
• Activity: Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid. The specific activity is 150,000pmoles/min/ug (Activity of this protein is much better than the world's famous brand) as measured under the described conditions .
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the C. Perfringens neuraminidase (P10481.1)(Cys 2-Gln 382) was expressed, with a N-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NA active protein

Background: Clostridium perfringens/C. perfringens (formerly known as C. welchii) is a Gram-positive, rod-shaped, anaerobic, spore-forming bacterium of the genus Clostridium. C. perfringens is ubiquitous in nature and can be found as a normal component of decaying vegetation, marine sediment, the intestinal tract of humans and other vertebrates, insects, and soil. C. perfringens is commonly encountered in infections as a benign component of the normal flora. In this case, its role in disease is minor. Infections due to C. perfringens show evidence of tissue necrosis, bacteremia, emphysematous cholecystitis, and gas gangrene, which is also known as clostridial myonecrosis. NA, also called sialidases, specifically catalyze the hydrolysis removal of terminal sialic acid residues from viral and cellular glycoconjugates. C. Perfringens neuraminidase catalyzes the hydrolysis of alpha-(2->3)-, alpha-(2->6)-, glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates, but has little activity against the alpha2-8 glycosidic linkages. The function of the neuraminidase is to release sialic acids for use as carbon and energy sources for the non-pathogenic bacterium, while in pathogenic microorganisms, sialidases have been suggested to be pathogenic factors

NCBI and Uniprot Product Information

• NCBI GI #: 127825
• UniProt Accession #: P10481
• Molecular Weight: 42,813 Da
• NCBI Official Full Name: Sialidase
• UniProt Protein Name: Sialidase
• Protein Family: Neuraminidase
• UniProt Gene Name: nanH
• UniProt Entry Name: NANH_CLOPF

Uniprot Description

Sialidases have been suggested to be pathogenic factors in microbial infections.