NEU2 recombinant protein

NEU2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121965

• Gene Names: NEU2; SIAL2
• Purity: >90% as determined by SDS-PAGE
• Synonyms: NEU2; NEU2 Protein; Human; Recombinant (His Tag); Human NEU2/Sialidase-2 Protein (His Tag); SIAL2 Protein; Neuraminidase 2; NEU2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 20% glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln380

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human NEU2 (CAB41449.1)(Met1-Gln380) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NEU2 recombinant protein

Background: Human sialidase 2 (NEU2) is a cytoplasmic sialidase that degrades sialyl glycoconjugates, including glycoproteins and gangliosides, via hydrolysis of terminal sialic acids to produce asialo-type molecules. It belongs to a family of glycohydrolytic enzymes that remove sialic acid residues from glycoproteins and glycolipids. NEU2 expression as assessed by quantitative real-time PCR was found to be extremely low or undetectable in many human tissues and cells, with notable exceptions like the placenta and testis. The human cytosolic sialidase NEU2 is the only mammalian enzyme structurally characterized and represents a valuable model to study the specificity of novel NA inhibitory drugs. Diseases associated with NEU2 include Galactosialidosis and Discrete Subaortic Stenosis.

NCBI and Uniprot Product Information

• NCBI GI #: 4688894
• NCBI GeneID: 4759
• UniProt Accession #: Q9Y3R4; Q3KNW4; Q6NTB4
• Molecular Weight: 42,254 Da
• NCBI Official Full Name: neuraminidase
• NCBI Official Synonym Full Names: neuraminidase 2
• NCBI Official Symbol: NEU2
• NCBI Official Synonym Symbols: SIAL2
• NCBI Protein Information: sialidase-2
• UniProt Protein Name: Sialidase-2
• Protein Family: Sialidase
• UniProt Gene Name: NEU2

NCBI Description

This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins, oligosaccharides and gangliosides.