Testing Data
Influenza A virus/Neuraminidase (NA) Active Protein | neuraminidase/NA active protein
Human CellExp Influenza A virus / Neuraminidase (NA)
Applications
SDS-Page
Purity
>=92%
Synonyms
Influenza A virus/Neuraminidase (NA); Human CellExp Influenza A virus / Neuraminidase (NA); NA; Neuraminidase; neuraminidase/NA active protein
Host
HEK293 cells
Purity/Purification
>=92%
Form/Format
Lyophilized from 0.22 mum filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Appearance: Lyophilized
Appearance: Lyophilized
Sequence Length
466
Applicable Applications for neuraminidase/NA active protein
SDS-PAGE
Solubility/Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
Endotoxin Level
<1 EU/mug by LAL method
Biological Activity
Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid. One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid per minute at pH 7.5 at 37 degree C
Results
Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid. One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid per minute at pH 7.5 at 37 degree C
Unit Definition
One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid per minute at pH 7.5 at 37 degree C
Handling
Centrifuge the vial prior to opening.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for neuraminidase/NA active protein
Background: Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.
Product Categories/Family for neuraminidase/NA active protein
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
Influenza A virus (A/Thailand/1(KAN-1)/2004 (H5N1)) Neuraminidase (NA) is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 46.1 kDa. The predicted N-terminus is His 36. DTT-reduced Protein migrates as 48 kDa in SDS-PAGE
NCBI Official Full Name
Neuraminidase
UniProt Protein Name
Neuraminidase
UniProt Gene Name
neuraminidase/NA
UniProt Entry Name
Q76UU8_9INFB
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Product Notes
The Influenza A virus/Neuraminidase (NA) neuraminidase/na (Catalog #AAA844917) is an Active Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Influenza A virus/Neuraminidase (NA) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the Influenza A virus/Neuraminidase (NA) neuraminidase/na for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Influenza A virus/Neuraminidase (NA), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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