Cathepsin Z/CTSZ Active Protein | CTSZ active protein

Cathepsin Z/CTSZ Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122526

• Gene Names: Ctsz; CTSX; AI787083; AU019819; D2Wsu143e
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Cathepsin Z/CTSZ; Cathepsin Z/CTSZ Protein; Mouse; Recombinant (His Tag); Mouse CTSZ/CTSX/Cathepsin Z Protein (His Tag); AI787083 Protein; AU019819 Protein; CTSX Protein; D2Wsu143e Protein; cathepsin Z; CTSZ active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Val306

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse CTSZ (NP_071720.1) precursor (Met 1-Val 306) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH. The specific activity is >1,200pmoles/min/ug.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CTSZ active protein

Background: Cathepsin Z (CTSZ), also known as Cathepsin X or CATX, belongs to the C1 family of lysosomal cysteine proteases. Its gene structure and activity properties show several unique features that distinguish it clearly from other human cysteine proteases. It has a very short pro-region that shows no similarity to those of other cathepsins and a three-residue insertion motif that forms a characteristic 'mini loop'. Cathepsin Z exhibits mono- and di-peptidase activity at its C-terminus, and in contrast to cathepsin B, it does not act as an endopeptidase. It is restricted to the cells of theimmune system, predominantly monocytes, macrophages and dendritic cells. Cathepsin Z is widely expressed in human tissues, suggesting that this enzyme could be involved in the normal intracellular protein degradation taking place in all cell types. It is capable to cleave regulatory motifs at C-terminus affecting the function of targeted molecules. Cathepsin X may regulate also the maturation of dendritic cells, a process, which is crucial in the initiation of adaptive immunity. Furthermore, higher levels of Cathepsin Z are also found in tumour and immune cells of prostate and gastric carcinomas and inmacrophages of gastric mucosa, especially after infection by Helicobacter pylori. Cathepsin Z is also ubiquitously distributed in cancer cell lines and in primary tumors from different sources, suggesting that this enzyme may participate in tumor progression.

References

Santamara I, et al. (1998) Cathepsin Z, a novel human cysteine proteinase with a short propeptide domain and a unique chromosomal location. J Biol Chem. 273(27): 16816-23.Kos J, et al. (2009) The role of cathepsin X in cell signaling. Cell Adh Migr. 3(2): 164-6.Sevenich L, et al. (2010) Synergistic antitumor effects of combined cathepsin B and cathepsin Z deficiencies on breast cancer progression and metastasis in mice. Proc Natl Acad Sci U S A. 107(6): 2497-502.

NCBI and Uniprot Product Information

• NCBI GI #: 11968166
• NCBI GeneID: 64138
• NCBI Accession #: NP_071720.1
• NCBI GenBank Nucleotide #: NM_022325.5
• UniProt Accession #: Q9WUU7
• Molecular Weight: 33,996 Da
• NCBI Official Full Name: cathepsin Z preproprotein
• NCBI Official Synonym Full Names: cathepsin Z
• NCBI Official Symbol: Ctsz
• NCBI Official Synonym Symbols: CTSX; AI787083; AU019819; D2Wsu143e
• NCBI Protein Information: cathepsin Z
• UniProt Protein Name: Cathepsin Z
• Protein Family: Cathepsin
• UniProt Gene Name: Ctsz
• UniProt Entry Name: CATZ_MOUSE

Uniprot Description

CTSZ: Exhibits carboxy-monopeptidase as well as carboxy- dipeptidase activity. Belongs to the peptidase C1 family.

Protein type: EC 3.4.18.1; Protease

Cellular Component: endoplasmic reticulum; extracellular space; intracellular membrane-bound organelle; lysosome

Molecular Function: cysteine-type endopeptidase activity

Biological Process: proteolysis involved in cellular protein catabolic process