Macrophage Inflammatory protein-1 beta Active Protein | MIP 1b active protein

Recombinant Human Macrophage Inflammatory protein-1 beta (CCL4)

Cat. Num. AAA142014

• Gene Names: CCL4; ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
• Purity: Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Macrophage Inflammatory protein-1 beta; Recombinant Human Macrophage Inflammatory protein-1 beta (CCL4); MIP 1b Human; Macrophage Inflammatory Protein-1 Beta Human Recombinant (CCL4); Small inducible cytokine A4; CCL4; Macrophage inflammatory protein 1-beta; MIP-1- beta; MIP-1-beta(1-69); T-cell activation protein 2; ACT-2; PAT 744; H400; SIS-gamma; Lymphocyte activation gene 1 protein; LAG-1; HC21; G-26 T-lymphocyte-secreted protein; chemokine (C-C motif) ligand 4; ACT2; G-26; LAG1; MIP1B; SCYA4; AT744.1; MGC104418; MGC126025; MGC126026; MIP 1b active protein

• Host: E Coli
• Purity/Purification: Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Pro-Met-Gly-Ser
• Sequence Length: 92

• Solubility: It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-1b in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The Activity is calculated by the ability to chemoattract Human blood monocytes using a concentration of 5-20ng/ml corresponding to a Specific Activity of 50,000-200,000IU/mg.
• Preparation and Storage: Lyophilized MIP-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CCL4 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for MIP 1b active protein

Description: Macrophage Inflammatory Protein-1 beta Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7620 Dalton. The CCL4 is purified by proprietary chromatographic techniques.

Introduction: Macrophage Inflammatory Proteins belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1a and MIP-1b that are now also named CCL3 and CCL4. Both factors are produced by macrophages after they are stimulated with bacterial endotoxins. MIP-1a and MIP-1b activate human granulocytes (neutrophils, eosinophils and basophils) which can lead to acute neutrophilic inflammation. MIP-1a and MIP-1b induce synthesis and release of other pro-inflammatory cytokines such as interleukin-1 (IL-1), IL-6 and TNF-alpha from fibroblasts and macrophages. CCL3 and CCL4 genes are both located on human chromosome 17.

Product Categories/Family for MIP 1b active protein

Chemokines; MIP

NCBI and Uniprot Product Information

• NCBI GI #: 4506845
• NCBI GeneID: 6351
• NCBI Accession #: NP_002975.1
• NCBI GenBank Nucleotide #: NM_002984.3
• UniProt Accession #: P13236; P22617; Q13704; Q3SXL8; Q6FGI8
• Molecular Weight: 10,212 Da
• NCBI Official Full Name: C-C motif chemokine 4
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 4
• NCBI Official Symbol: CCL4
• NCBI Official Synonym Symbols: ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
• NCBI Protein Information: C-C motif chemokine 4; G-26 T-lymphocyte-secreted protein; MIP-1-beta(1-69); PAT 744; SIS-gamma; T-cell activation protein 2; lymphocyte activation gene 1 protein; macrophage inflammatory protein 1-beta; secreted protein G-26; small inducible cytokine A4 (homologous to mouse Mip-1b)
• UniProt Protein Name: C-C motif chemokine 4
• UniProt Gene Name: CCL4
• UniProt Synonym Gene Names: LAG1; MIP1B; SCYA4; LAG-1; MIP-1-beta; ACT-2
• UniProt Entry Name: CCL4_HUMAN

NCBI Description

The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. The encoded protein is secreted and has chemokinetic and inflammatory functions. [provided by RefSeq, Dec 2012]

Uniprot Description

CCL4: Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5- mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; extracellular region

Molecular Function: identical protein binding; protein binding; CCR1 chemokine receptor binding; chemokine activity; cytokine activity; CCR5 chemokine receptor binding

Biological Process: cell-cell signaling; response to toxin; response to virus; establishment and/or maintenance of cell polarity; positive regulation of calcium-mediated signaling; immune response; signal transduction; inflammatory response; positive regulation of calcium ion transport; cell adhesion; cell motility