Human C-C motif chemokine 4 ELISA Kit | CCL4 elisa kit

Human C-C motif chemokine 4 ELISA Kit

Cat. Num. AAA765945

• Gene Names: CCL4; ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
• Reactivity: Human
• Synonyms: C-C motif chemokine 4; Human C-C motif chemokine 4 ELISA Kit; CCL4/MIP-1b/ACT2/AT744.1/G-26/HC21/LAG-1/LAG1/MIP-1-beta/MIP1B1/SCYA2/SCYA4; CCL4 elisa kit

• Reactivity: Human
• Sequence Length: 92

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.2-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CCL4 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CCL4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CCL4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CCL4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CCL4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4506845
• NCBI GeneID: 6351
• NCBI Accession #: NP_002975.1
• NCBI GenBank Nucleotide #: NM_002984.3
• Molecular Weight: 10,212 Da
• NCBI Official Full Name: C-C motif chemokine 4
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 4
• NCBI Official Symbol: CCL4
• NCBI Official Synonym Symbols: ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
• NCBI Protein Information: C-C motif chemokine 4
• UniProt Protein Name: C-C motif chemokine 4
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL4
• UniProt Synonym Gene Names: LAG1; MIP1B; SCYA4; LAG-1; MIP-1-beta; ACT-2
• UniProt Entry Name: CCL4_HUMAN

NCBI Description

The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. The encoded protein is secreted and has chemokinetic and inflammatory functions. [provided by RefSeq, Dec 2012]

Uniprot Description

CCL4: Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5- mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Chemokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular region; extracellular space

Molecular Function: CCR1 chemokine receptor binding; CCR5 chemokine receptor binding; chemokine activity; cytokine activity; identical protein binding; protein binding

Biological Process: cell adhesion; cell motility; cell-cell signaling; establishment and/or maintenance of cell polarity; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; lymphocyte chemotaxis; monocyte chemotaxis; neutrophil chemotaxis; positive regulation of calcium ion transport; positive regulation of calcium-mediated signaling; positive regulation of GTPase activity; positive regulation of inflammatory response; positive regulation of tumor necrosis factor production; positive regulation of vascular permeability; response to toxin; response to virus; signal transduction