Rabbit AKAP 1 Polyclonal Antibody | anti-AKAP1 antibody

AKAP 1 (N306) Polyclonal Antibody

Cat. Num. AAA3002696

• Gene Names: AKAP1; AKAP; PRKA1; AKAP84; TDRD17; AKAP121; AKAP149; D-AKAP1; PPP1R43; SAKAP84
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: AKAP 1; Polyclonal Antibody; AKAP 1 (N306) Polyclonal Antibody; A-kinase anchor protein 1; mitochondrial; A-kinase anchor protein 149 kDa; AKAP 149; Dual specificity A-kinase-anchoring protein 1; D-AKAP-1; Protein kinase A-anchoring protein 1; PRKA1; Spermatid A-kinase anchor protein 84; S-AKAP84; AKAP1; AKAP149; AKAP-1; AKAP-149; anti-AKAP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: AKAP 1 (N306) polyclonal antibody detects endogenous levels of AKAP 1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 903

• Applicable Applications for anti-AKAP1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 274-320 of Human AKAP 1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of AKAP 1 (N306) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:SK-OVCAR3 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of AKAP 1 (N306) pAb in paraffin-embedded human brain tissue.)

Western Blot (WB)

(Western Blot (WB) analysis of AKAP1 (N306) pAb at 1:500 dilutionLane1:MCF-7 whole cell lysate (40ug) Lane2:SK-OVCAR3 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:The testis tissue lysate of Mouse (40ug) Lane5:The testis tissue lysate of Rat (40ug))

Related Product Information for anti-AKAP1 antibody

AKAP1, also known as AKAP149 in human, AKAP121 in rat, or D-AKAP in mouse is a dual-specificity AKAP which can bind to both RI and RII subunits of PKA with similar affinity. Originally thought to be predominantly restricted to the mitochondria, growing evidence suggests that localization of AKAP1 can be regulated in part by alternative splicing events and that AKAP1 may be present in the endoplasmic reticulum-nuclear envelope membrane network. Peri-nuclear localization, along with the fact that AKAP1 interacts with RNA via one of two nucleotide-binding domains (K homology (KH) and Tudor) have lead some to suggest that AKAP1 may play a role in RNA metabolism. In addition to PKA-RI and -RII, AKAP1 directly interacts with PP1 in a phosphorylation dependent manner and nucleates a complex containing PP2Ac, PKA and RSK1 which modulates RSK1 localization and activity.

NCBI and Uniprot Product Information

• NCBI GI #: 339275974
• NCBI GeneID: 8165
• NCBI Accession #: NP_001229831.1
• NCBI GenBank Nucleotide #: NP_001229831.1
• UniProt Accession #: Q92667
• Molecular Weight: ~ 90 to 150kDa
• NCBI Official Full Name: A-kinase anchor protein 1, mitochondrial
• NCBI Official Synonym Full Names: A-kinase anchoring protein 1
• NCBI Official Symbol: AKAP1
• NCBI Official Synonym Symbols: AKAP; PRKA1; AKAP84; TDRD17; AKAP121; AKAP149; D-AKAP1; PPP1R43; SAKAP84
• NCBI Protein Information: A-kinase anchor protein 1, mitochondrial
• UniProt Protein Name: A-kinase anchor protein 1, mitochondrial
• Protein Family: A-kinase anchor protein
• UniProt Gene Name: AKAP1
• UniProt Synonym Gene Names: AKAP149; PRKA1; AKAP 149; D-AKAP-1; PRKA1; S-AKAP84
• UniProt Entry Name: AKAP1_HUMAN

NCBI Description

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq, Jul 2008]

Uniprot Description

AKAP1: Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. AKAP149 is highly expressed in prostate and small intestine whereas S-AKAP84 is expressed in kidney, pancreas, liver, lung and brain. AKAP149 is also expressed in colon carcinoma. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Membrane protein, integral; Mitochondrial; RNA-binding

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: mitochondrial outer membrane; membrane; mitochondrion; integral to membrane; cytosol

Molecular Function: protein binding; RNA binding

Biological Process: blood coagulation