Rabbit MIP-1 BETA Polyclonal Antibody | anti-MIP-1 BETA antibody

RABBIT ANTI HUMAN MIP-1 BETA

Cat. Num. AAA221343

• Gene Names: CCL4; ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
• Applications: ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: MIP-1 BETA; Polyclonal Antibody; RABBIT ANTI HUMAN MIP-1 BETA; anti-MIP-1 BETA antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 92

• Applicable Applications for anti-MIP-1 BETA antibody: ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: ELISA: Maximum Dilution: 0.5ug/ml;; Functional Assays: Minimum Dilution: 1.75; Maximum Dilution: 3.0ug/ml;; Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; ; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
• Immunogen: Recombinant human MIP-1 beta.; Buffer Solution
• Antiserum Preparation: Antisera to human MIP-1 beta were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Formalin fixed, paraffin embedded human pancreatic adenocarcinoma stained with Rabbit anti human MIP-1β (MBS221343) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Related Product Information for anti-MIP-1 BETA antibody

Rabbit anti Human MIP-1 beta antibody recognizes human MIP-1 beta, otherwise known as CCL4, a 69 amino acid member of the CC chemokine family, shown to signal through the chemokine receptors CCR1, CCR2 and CCR5. MIP-1 beta is involved in host defence against invading pathogens, acting as a regulator of activation and trafficking of inflammatory cells, such as monocytes, B cells, NK cells, neutrophils, dendritic cells and CD4+ lymphocytes. The secretion of MIP-1 beta by CD8+ lymphocytes has been shown to act as a major HIV-suppressive factor (HIV-SF) through binding to CCR5, identified as a coreceptor for HIV-1 target cell entry.

NCBI and Uniprot Product Information

• NCBI GI #: 4506845
• NCBI GeneID: 6351
• NCBI Accession #: NP_002975.1
• NCBI GenBank Nucleotide #: NM_002984.3
• UniProt Accession #: P13236; P22617; Q13704; Q3SXL8; Q6FGI8
• Molecular Weight: 10,212 Da
• NCBI Official Full Name: C-C motif chemokine 4
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 4
• NCBI Official Symbol: CCL4
• NCBI Official Synonym Symbols: ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
• NCBI Protein Information: C-C motif chemokine 4; G-26 T-lymphocyte-secreted protein; MIP-1-beta(1-69); PAT 744; SIS-gamma; T-cell activation protein 2; lymphocyte activation gene 1 protein; macrophage inflammatory protein 1-beta; secreted protein G-26; small inducible cytokine A4 (homologous to mouse Mip-1b)
• UniProt Protein Name: C-C motif chemokine 4
• UniProt Gene Name: CCL4
• UniProt Synonym Gene Names: LAG1; MIP1B; SCYA4; LAG-1; MIP-1-beta; ACT-2
• UniProt Entry Name: CCL4_HUMAN

NCBI Description

The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. The encoded protein is secreted and has chemokinetic and inflammatory functions. [provided by RefSeq, Dec 2012]

Uniprot Description

CCL4: Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5- mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; extracellular region

Molecular Function: identical protein binding; CCR1 chemokine receptor binding; protein binding; chemokine activity; cytokine activity; CCR5 chemokine receptor binding

Biological Process: cell-cell signaling; response to virus; response to toxin; establishment and/or maintenance of cell polarity; immune response; positive regulation of calcium-mediated signaling; signal transduction; positive regulation of calcium ion transport; cell adhesion; inflammatory response; cell motility