Human CCL4/MIP-1 beta ELISA Kit | CCL4 elisa kit

Human CCL4/MIP-1 beta PicoKine ELISA Kit

Cat. Num. AAA176557

• Gene Names: CCL4L1; LAG1; CCL4L; LAG-1; SCYA4L; AT744.2; SCYA4L1
• Reactivity: Human
• Synonyms: CCL4/MIP-1 beta; Human CCL4/MIP-1 beta PicoKine ELISA Kit; C-C motif chemokine 4; chemokine (C-C motif) ligand 4; ACT 2; ACT2; AT744.1; C C motif chemokine 4; CCL4; CCL4L1; Chemokine CC Motif Ligand 4; G 26 T lymphocyte-secreted protein; HC21; LAG 1; LAG1; Lymphocyte activation gene 1; Lymphocyte activation gene 1 protein; MGC104418; MGC126025; MGC126026; MIP 1 beta; MIP1B; PAT 744; Protein H400; SCYA2; SCYA4; Secreted protein G 26; SIS gamma; Small inducible cytokine A4; T cell activation protein 2; CCL4 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human CCL4
• Sequence Length: 92

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Assay Type: Sandwich
• Detection Range: 7.8pg/ml-500pg/ml
• Sensitivity: < 1pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human CCL4/MIP-1 beta PicoKine ELISA Kit standard curve )

Related Product Information for CCL4 elisa kit

Principle of the assay: human CCL4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL4 has been precoated onto 96-well plates. Standards(E.coli, A24-N92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL4 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in humans is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. the CCL4 gene was assigned to a slightly more distal location: 17q21-q23 rather than 17q11-q21.

NCBI and Uniprot Product Information

• NCBI GI #: 127080
• NCBI GeneID: 388372
• UniProt Accession #: P13236; P22617; Q13704; Q3SXL8; Q6FGI8
• Molecular Weight: 10,212 Da
• NCBI Official Full Name: C-C motif chemokine 4
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 4-like 1
• NCBI Official Symbol: CCL4L1
• NCBI Official Synonym Symbols: LAG1; CCL4L; LAG-1; SCYA4L; AT744.2; SCYA4L1
• NCBI Protein Information: C-C motif chemokine 4-like; CC chemokine ligand 4L1; lymphocyte activation gene 1; small inducible cytokine A4-like; macrophage inflammatory protein-1b2; chemokine (C-C motif) ligand 4-like 1, telomeric
• UniProt Protein Name: C-C motif chemokine 4
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL4
• UniProt Synonym Gene Names: LAG1; MIP1B; SCYA4; LAG-1; MIP-1-beta; ACT-2
• UniProt Entry Name: CCL4_HUMAN

NCBI Description

This gene is one of several cytokine genes that are clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins that function in inflammatory and immunoregulatory processes. The protein encoded by this family member is similar to the chemokine (C-C motif) ligand 4 product, which inhibits HIV entry by binding to the cellular receptor CCR5. The copy number of this gene varies among individuals, where most individuals have one to five copies. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

CCL4: Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5- mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; extracellular region

Molecular Function: identical protein binding; protein binding; CCR1 chemokine receptor binding; chemokine activity; cytokine activity; CCR5 chemokine receptor binding

Biological Process: cell-cell signaling; response to virus; response to toxin; establishment and/or maintenance of cell polarity; positive regulation of calcium-mediated signaling; immune response; inflammatory response; signal transduction; cell adhesion; cell motility; positive regulation of calcium ion transport