Monocyte Chemotactic Protein-5 Active Protein | MCP 5 active protein

Recombinant Mouse Monocyte Chemotactic Protein-5 (CCL12)

Cat. Num. AAA145071

• Gene Names: Ccl12; MCP-5; Scya12
• Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Monocyte Chemotactic Protein-5; Recombinant Mouse Monocyte Chemotactic Protein-5 (CCL12); MCP 5 Mouse; Monocyte Chemotactic Protein-5 Mouse Recombinant (CCL12); C-C motif chemokine 12; MCP-1-related chemokine; Monocyte chemoattractant protein 5; Monocyte chemotactic protein 5; MCP-5; Small-inducible cytokine A12; Ccl12; Mcp5; Scya12; MCP 5 active protein

• Host: E Coli
• Purity/Purification: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 um filtered concentrated solution in 1xPBS, pH 7.4.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG
• Sequence Length: 104

• Solubility: It is recommended to reconstitute the lyophilized MCP5 in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 10.0-50.0 ng/ml.
• Preparation and Storage: Lyophilized CCL12 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution MCP5 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for MCP 5 active protein

Description: MCP-5 Mouse Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 82 amino acids and having a molecular mass of 9.3kDa. The MCP5 is purified by proprietary chromatographic techniques.

Introduction: MCP-5 (CCL12) is a cloned mouse CC chemokine most closely related to human MCP1 (66% amino acid sequence identity in the mature protein). MCP5 is expressed constitutively in the thymus and the lymph nodes. Under inflammatory conditions, moreover CCL12 expression is induced in activated macrophages and mast cells. The mouse MCP1 gene is mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 is a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, MCP-5 also chemoattracts eosinophils. CCL12 is a functional ligand for CCR2.

Product Categories/Family for MCP 5 active protein

Chemokines; MCP

NCBI and Uniprot Product Information

• NCBI GI #: 119226206
• NCBI GeneID: 20293
• NCBI Accession #: NP_035461.2
• NCBI GenBank Nucleotide #: NM_011331.2
• UniProt Accession #: Q62401; Q9QYD6
• Molecular Weight: 11,659 Da
• NCBI Official Full Name: C-C motif chemokine 12
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 12
• NCBI Official Symbol: Ccl12
• NCBI Official Synonym Symbols: MCP-5; Scya12
• NCBI Protein Information: C-C motif chemokine 12; MCP-1-related chemokine; monocyte chemoattractant protein 5; monocyte chemotactic protein 5; small inducible cytokine A12; small-inducible cytokine A12
• UniProt Protein Name: C-C motif chemokine 12
• UniProt Gene Name: Ccl12
• UniProt Synonym Gene Names: Mcp5; Scya12; MCP-5
• UniProt Entry Name: CCL12_MOUSE

Uniprot Description

CCL2: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region; intracellular

Molecular Function: chemokine activity; CCR2 chemokine receptor binding; cytokine activity

Biological Process: cytokine and chemokine mediated signaling pathway; positive regulation of leukocyte migration; MAPKKK cascade; cytoskeleton organization and biogenesis; chemotaxis; macrophage chemotaxis; monocyte chemotaxis; regulation of cell shape; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; immune response; negative regulation of neuron apoptosis; angiogenesis; inflammatory response; astrocyte cell migration