MCP-5 Recombinant Protein | Ccl12 recombinant protein

MCP-5 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122472

• Gene Names: Ccl12; MCP-5; Scya12
• Purity: >90% as determined by SDS-PAGE
• Synonyms: MCP-5; MCP-5 Protein; Mouse; Recombinant (His Tag); Mouse CCL12/MCP-5 Protein (His Tag); CCL12 Protein; Scya12 Protein; chemokine (C-C motif) ligand 12; Ccl12 recombinant protein

• Host: Yeast
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly23-Gly104

• Species: Mouse
• Predicted N Terminal: Gly 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse Ccl12 (NP_035461.2)(Gly23-Gly104) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for Ccl12 recombinant protein

Background: Ccl12 prevented initiation of the reparative response by prolonging inflammation and inhibiting fibroblast conversion to myofibroblasts, resulting in diminished scar formation. Macrophage secretion of Ccl12 directly impaired fibronectin and collagen deposition and indirectly stimulated collagen degradation through upregulation of matrix metalloproteinase-2. In post-MI patients, circulating LPS levels strongly associated with the Ccl12 homologue monocyte chemotactic protein 1 (MCP-1). Both MCP-1 and MCP-5 are HIF-1 target genes and that HIF-1alpha is involved in transcriptional induction of these two chemokines in astrocytes by hypoxia.

NCBI and Uniprot Product Information

• NCBI GI #: 119226206
• NCBI GeneID: 20293
• NCBI Accession #: NP_035461.2
• NCBI GenBank Nucleotide #: NM_011331.2
• UniProt Accession #: Q62401; Q9QYD6
• Molecular Weight: 11,659 Da
• NCBI Official Full Name: C-C motif chemokine 12
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 12
• NCBI Official Symbol: Ccl12
• NCBI Official Synonym Symbols: MCP-5; Scya12
• NCBI Protein Information: C-C motif chemokine 12; MCP-1-related chemokine; monocyte chemoattractant protein 5; monocyte chemotactic protein 5; small inducible cytokine A12; small-inducible cytokine A12
• UniProt Protein Name: C-C motif chemokine 12
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl12
• UniProt Synonym Gene Names: Mcp5; Scya12; MCP-5
• UniProt Entry Name: CCL12_MOUSE

Uniprot Description

CCL2: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region; intracellular

Molecular Function: chemokine activity; CCR2 chemokine receptor binding; cytokine activity

Biological Process: cytokine and chemokine mediated signaling pathway; positive regulation of leukocyte migration; MAPKKK cascade; cytoskeleton organization and biogenesis; chemotaxis; macrophage chemotaxis; monocyte chemotaxis; regulation of cell shape; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; immune response; negative regulation of neuron apoptosis; angiogenesis; inflammatory response; astrocyte cell migration