Testing Data
(Anti-TdT Picoband antibody, MBS177416-1.jpgAll lanes: Anti TDT (MBS177416) at 0.5ug/mlWB: Recombinant Human TDT Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD )
Rabbit DNA nucleotidylexotransferase Polyclonal Antibody | anti-DNTT antibody
Anti-TdT Antibody
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Testing Data
(Anti-TdT Picoband antibody, MBS177416-1.jpgAll lanes: Anti TDT (MBS177416) at 0.5ug/mlWB: Recombinant Human TDT Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD )
Testing Data
(Anti-TdT Picoband antibody, MBS177416-1.jpgAll lanes: Anti TDT (MBS177416) at 0.5ug/mlWB: Recombinant Human TDT Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD )
Western Blot (WB)
(Anti-TdT Picoband antibody, MBS177416-2.jpgAll lanes: Anti TDT (MBS177416) at 0.5ug/mlWB: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 58KDObserved bind size: 58KD )
Western Blot (WB)
(Anti-TdT Picoband antibody, MBS177416-2.jpgAll lanes: Anti TDT (MBS177416) at 0.5ug/mlWB: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 58KDObserved bind size: 58KD )
Background: Terminal Deoxynucleotidyl Transferase, also known as TDT and terminal transferase, is a unique DNA polymerase without template direction catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers. Its gene is mapped to the region 10q23-q24. And TDT cDNA contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids. TDT may be responsible for inserting nucleotides (N regions) at the V(H)-D and D-J(H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TDT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
DNTT: Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. Belongs to the DNA polymerase type-X family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.7.31; Transferase
Chromosomal Location of Human Ortholog: 10q23-q24
Cellular Component: nucleoplasm; cytoplasm
Molecular Function: DNA binding; metal ion binding; DNA-directed DNA polymerase activity; DNA nucleotidylexotransferase activity
Biological Process: DNA modification
Research Articles on DNTT
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Product Notes
The DNTT dntt (Catalog #AAA177416) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TdT Antibody reacts with Human. No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's DNA nucleotidylexotransferase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: Concentration: 0.1-0.5mug/ml; Tested Species: Human. Researchers should empirically determine the suitability of the DNTT dntt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA nucleotidylexotransferase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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