GRO gamma (CXCL3) Active Protein | CXCL3 active protein

Recombinant Mouse GRO gamma (CXCL3)

Cat. Num. AAA4159957

• Gene Names: Cxcl3; Dcip1; Gm1960
• Purity: >95% as determined by SDS-PAGE
• Synonyms: GRO gamma (CXCL3); Recombinant Mouse GRO gamma (CXCL3); C-X-C motif chemokine 3; Dendritic cell inflammatory protein 1; Dcip1; Gm1960; Recombinant Mouse Growth-Regulated Oncogene Gamma (CXCL3); CXCL3 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution in Phosphate and NaCl
• Sequence Length: 100

• Bioactivity: Determined by its ability to chemoattract 293 transfected CXCR2 cells using a concentration range of 10.0-100 ng/mL.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Related Product Information for CXCL3 active protein

Growth regulated oncogene-gamma belongs to the family of chemotyctic cytokines called chemokines. It is identical with MGSA (melanoma growth stimulatory activity) and the new designation is CXCL3. This factor is known mainly because of its chemotactic activity. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

NCBI and Uniprot Product Information

• NCBI GI #: 42600573
• NCBI GeneID: 330122
• NCBI Accession #: NP_976065.1
• NCBI GenBank Nucleotide #: NP_976065.1
• UniProt Accession #: Q6W5C0
• Molecular Weight: 8.0 kDa
• NCBI Official Full Name: C-X-C motif chemokine 3
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 3
• NCBI Official Symbol: Cxcl3
• NCBI Official Synonym Symbols: Dcip1; Gm1960
• NCBI Protein Information: C-X-C motif chemokine 3
• UniProt Protein Name: C-X-C motif chemokine 3
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: Cxcl3
• UniProt Entry Name: CXCL3_MOUSE

NCBI Description

This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. [provided by RefSeq, May 2013]

Uniprot Description

CXCL1: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular space; cell; extracellular region; cytosol

Molecular Function: protein binding; chemokine activity; CXCR chemokine receptor binding; cytokine activity

Biological Process: G-protein coupled receptor protein signaling pathway; neutrophil chemotaxis; elevation of cytosolic calcium ion concentration; positive regulation of leukocyte chemotaxis; immune response; response to lipopolysaccharide; chemotaxis; inflammatory response; regulation of cell proliferation