GRO gamma (CXCL3) Recombinant Protein | GROgamma recombinant protein

GRO gamma (CXCL3)

Cat. Num. AAA517038

• Applications: Cell Assay
• Synonyms: GRO gamma (CXCL3); C-X-C motif chemokine 3; GRO-gamma(1-73); Growth-regulated protein gamma; GRO-gamma; Macrophage inflammatory protein 2-beta; MIP2-beta Recombinant Human Growth Regulated Oncogene Gamma (CXCL3); GROgamma recombinant protein

• Host: E Coli
• Form/Format: Recombinant GRO-gamma/CXCL3 was lyophilized from a 0.2 um filtered concentrated (1.0mg/ml) solution in 40mM NaCl, 10mM PB, pH7.0.

• Applicable Applications for GROgamma recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1mg/ml. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Activity

(Sample Cell Assay Data: Determined by its ability to chemoattract 293 transfected CXCR2 cells using a concentration range of 10-100 ng/mL.)

SDS-Page

(Purity: Recombinant Human GRO gamma (CXCL3) resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.)

Related Product Information for GROgamma recombinant protein

Recombinant Human GRO gamma (CXCL3) was expressed in E Coli cells. The protein accession number is P19876
Growth regulated oncogene-gamma belongs to the family of chemotyctic cytokines called chemokines. It is identical with MGSA (melanoma growth stimulatory activity) and the new designation is CXCL3. This factor is known mainly because of its chemotactic activity. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

Product Categories/Family for GROgamma recombinant protein

Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 355749325
• UniProt Accession #: P19876; Q4W5H9
• Molecular Weight: 11,342 Da
• NCBI Official Full Name: Macrophage inflammatory protein 2-beta
• UniProt Protein Name: C-X-C motif chemokine 3
• UniProt Gene Name: CXCL3
• UniProt Synonym Gene Names: GRO3; GROG; SCYB3; GRO-gamma; MIP2-beta
• UniProt Entry Name: CXCL3_HUMAN

Uniprot Description

CXCL3: Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; neutrophil chemotaxis; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; immune response; inflammatory response; regulation of cell proliferation