Mouse GRO gamma ELISA Kit | CXCL3 elisa kit

Mouse GRO gamma ELISA Kit

Cat. Num. AAA8248429

• Gene Names: Cxcl3; Dcip1; Gm1960
• Reactivity: Mouse
• Synonyms: GRO gamma; Mouse GRO gamma ELISA Kit; GRO3; GROG; SCYB3; C-X-C motif chemokine 3; GRO-gamma(1-73); Growth-regulated protein gamma; GRO-gamma; Macrophage inflammatory protein 2-beta; MIP2-beta; CXCL3 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse GRO gamma ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse GRO gamma proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 100

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: 10 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CXCL3 elisa kit

Principle of the Assay: The Mouse GRO gamma ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse GRO gamma in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse GRO gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and GRO gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse GRO gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GRO gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Chemokine (C-X-C motif) ligand 3 (CXCL3) is a small cytokine belonging to the CXC chemokine family. It is mapped to 14p2 in rat. CXCL3 controls migration and adhesion of monocytes and mediates its effects on its target cell by interacting with a cell surface chemokine receptor. It has been shown that CXCL3 regulates cell autonomously the migration of the precursors of cerebellar granule neurons toward the internal layers of cerebellum, during the morphogenesis of cerebellum. CXCL3 also play fundamental roles in the development, homeostasis and it has effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.

NCBI and Uniprot Product Information

• NCBI GI #: 42600573
• NCBI GeneID: 330122
• NCBI Accession #: NP_976065.1
• NCBI GenBank Nucleotide #: NM_203320.3
• UniProt Accession #: Q6W5C0; Q3UNK9
• Molecular Weight: 10,686 Da
• NCBI Official Full Name: C-X-C motif chemokine 3
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 3
• NCBI Official Symbol: Cxcl3
• NCBI Official Synonym Symbols: Dcip1; Gm1960
• NCBI Protein Information: C-X-C motif chemokine 3
• UniProt Protein Name: C-X-C motif chemokine 3
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: Cxcl3
• UniProt Entry Name: CXCL3_MOUSE

NCBI Description

This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. [provided by RefSeq, May 2013]

Uniprot Description

CXCL1: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Cellular Component: extracellular space

Molecular Function: chemokine activity; CXCR chemokine receptor binding; protein binding

Biological Process: elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; neutrophil chemotaxis; regulation of cell proliferation; response to lipopolysaccharide