CXCL1 active protein

Recombinant Human CXCL1

Cat. Num. AAA4159658

• Gene Names: CXCL1; FSP; GRO1; GROa; MGSA; NAP-3; SCYB1; MGSA-a
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CXCL1; Recombinant Human CXCL1; Growth Regulated Protein; Melanoma Growth Stimulatory Activity; GRO-alpha; MGSA-alpha; NAP-3; GRO1; C-X-C motif chemokine 1; GRO-alpha(1-73); Recombinant Human Growth Regulated Alpha Protein (CXCL1); CXCL1 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution in 40 mM NaCl and PB, pH 7.0
• Sequence Length: 107

• Bioactivity: Determined by the ability to chemoattract human neutrophils using a concentration range of 10.0-50.0 ng/mL
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Related Product Information for CXCL1 active protein

A chemokine, the growth regulated oncogene-alpha is identical with MGSA (melanoma growth stimulatory activity) with its new designation being CXCL1. The expression of GRO can be induced by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

NCBI and Uniprot Product Information

• NCBI GI #: 4504153
• NCBI GeneID: 2919
• NCBI Accession #: NP_001502.1
• NCBI GenBank Nucleotide #: NP_001502.1
• UniProt Accession #: P09341
• Molecular Weight: 8.0 kDa
• NCBI Official Full Name: growth-regulated alpha protein
• NCBI Official Synonym Full Names: C-X-C motif chemokine ligand 1
• NCBI Official Symbol: CXCL1
• NCBI Official Synonym Symbols: FSP; GRO1; GROa; MGSA; NAP-3; SCYB1; MGSA-a
• NCBI Protein Information: growth-regulated alpha protein
• UniProt Protein Name: Growth-regulated alpha protein
• Protein Family: Growth-regulated alpha protein
• UniProt Gene Name: CXCL1
• UniProt Synonym Gene Names: GRO; GRO1; GROA; MGSA; SCYB1; MGSA; NAP-3
• UniProt Entry Name: GROA_HUMAN

NCBI Description

This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL1: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO- alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; chemokine activity; CXCR chemokine receptor binding; enzyme activator activity; receptor binding

Biological Process: positive regulation of catalytic activity; negative regulation of cell proliferation; cell proliferation; nervous system development; G-protein coupled receptor protein signaling pathway; immune response; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; inflammatory response; actin cytoskeleton organization and biogenesis; chemotaxis; signal transduction