DUSP21 active protein

DUSP21, human recombinant

Cat. Num. AAA844230

• Gene Names: DUSP21; LMWDSP21
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: DUSP21; human recombinant; Dual specificity protein phosphatase 21; DUSP21 active protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: 0.25 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 200 mM NaCl and 2 mM DTT; Appearance: Liquid
• Concentration: 0.25 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMTASAS SFSSSQGVQQ PSIYSFSQIT RSLFLSNGVA ANDKLLLSSN RITAIVNASV EVVNVFFEGI QYIKVPVTDA RDSRLYDFFD PIADLIHTID MRQGRTLLHC MAGVSRSASL CLAYLMKYHS MSLLDAHTWT KSRRPIIRPN NGFWEQLINY EFKLFNNNTV RMINSPVGNI PDIYEKDLRM MISM
• Sequence Length: 190

• Applicable Applications for DUSP21 active protein: SDS-PAGE
• Biological Activity: Specific activity is > 1800 unit/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37 degree C, pH7.5 using 10 mM of substrate.
• Results: Specific activity is > 1800 unit/mg
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for DUSP21 active protein

Background: DuSP21 (Dual specificity protein phosphatase 21) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases (DuSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. This enzyme can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. It is exclusively expressed in testis where is preferentially dephosphorylates phosphotyrosine residues in MAPK peptides. Recombinant human DuSP21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family for DUSP21 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 225703077
• NCBI GeneID: 63904
• NCBI Accession #: NP_071359.3
• NCBI GenBank Nucleotide #: NM_022076.3
• UniProt Accession #: Q9H596; Q0VDA6; Q6IAJ6; Q6YDQ8
• Molecular Weight: 24.1 kDa (214 aa, 1-190 aa + His Tag)
• NCBI Official Full Name: dual specificity protein phosphatase 21
• NCBI Official Synonym Full Names: dual specificity phosphatase 21
• NCBI Official Symbol: DUSP21
• NCBI Official Synonym Symbols: LMWDSP21
• NCBI Protein Information: dual specificity protein phosphatase 21
• UniProt Protein Name: Dual specificity protein phosphatase 21
• Protein Family: Dual specificity phosphatase
• UniProt Gene Name: DUSP21
• UniProt Synonym Gene Names: LMWDSP21; LMW-DSP21
• UniProt Entry Name: DUS21_HUMAN

NCBI Description

This gene encodes a member of the dual specificity phosphatase family, specifically the low molecular weight dual specificity phosphatase family. The encoded protein localizes to both the cytoplasm and the nucleus and functions to remove phosphate groups from phosphotyrosine and phosphothreonine residues.[provided by RefSeq, Mar 2009]

Uniprot Description

DUSP21: Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Protein type: Protein phosphatase, dual-specificity; EC 3.1.3.16; Motility/polarity/chemotaxis; Mitochondrial; EC 3.1.3.48

Chromosomal Location of Human Ortholog: Xp11.4-p11.23

Cellular Component: mitochondrial matrix; cytoplasm; nucleus

Molecular Function: protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process: inactivation of MAPK activity