PTPN7 active protein

PTPN7, human recombinant

Cat. Num. AAA844776

• Gene Names: PTPN7; LPTP; HEPTP; PTPNI; BPTP-4; LC-PTP
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: PTPN7; human recombinant; Tyrosine-protein phosphatase non-receptor type 7; BPTP-4; HEPTP; LC-PTP; LPTP; PTPNI; PTPN7 active protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 M NaCl and 1 mM DTT; Appearance: Liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMVQAHG GRSRAQPLTL SLGAAMTQPP PEKTPAKKHV RLQERRGSNV ALMLDVRSLG AVEPICSVNT PREVTLHFLR TAGHPLTRWA LQRQPPSPKQ LEEEFLKIPS NFVSPEDLDI PGHASKDRYK TILPNPQSRV CLGRAQSQED GDYINANYIR GYDGKEKVYI ATQGPMPNTV SDFWEMVWQE EVSLIVMLTQ LREGKEKCVH YWPTEEETYG PFQIRIQDMK ECPEYTVRQL TIQYQEERRS VKHILFSAWP DHQTPESAGP LLRLVAEVEE SPETAAHPGP IVVHCSAGIG RTGCFIATRI GCQQLKARGE VDILGIVCQL RLDRGGMIQT AEQYQFLHHT LALYAGQLPE EPSP
• Sequence Length: 465

• Applicable Applications for PTPN7 active protein: SDS-PAGE
• Biological Activity: Specific activity is > 2000 unit/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37 degree C, pH7.5 using 10 mM of substrate.
• Results: Specific activity is > 2000 unit/mg
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for PTPN7 active protein

Background: PTPN7, also known as tyrosine-protein phosphatase non-receptor type 7 isoform 1, acts preferentially on tyrosine-phosphorylated MAPK1. This protein plays a role in the regulation of T and B-lymphocyte development and signal transduction. Recombinant human PTPN7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Product Categories/Family for PTPN7 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 315221140
• NCBI GeneID: 5778
• NCBI Accession #: NP_002823.3
• NCBI GenBank Nucleotide #: NM_002832.3
• UniProt Accession #: P35236; Q53XK4; Q5SXQ0; Q5SXQ1; Q9BV05; B3KXE1
• Molecular Weight: 43.1 kDa (384 aa, 1-360 aa + His Tag)
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 7 isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 7
• NCBI Official Symbol: PTPN7
• NCBI Official Synonym Symbols: LPTP; HEPTP; PTPNI; BPTP-4; LC-PTP
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 7
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 7
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: PTPN7
• UniProt Synonym Gene Names: HEPTP
• UniProt Entry Name: PTN7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This gene is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. The non-catalytic N-terminus of this PTP can interact with MAP kinases and suppress the MAP kinase activities. This PTP was shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

HePTP: a non-receptor protein-tyrosine phosphatase. Preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. Its noncatalytic N-terminus can interact with MAP kinases and suppress the MAP kinase activities. Shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway. Three alternatively spliced transcript variants of this gene, which encode two distinct isoforms, are reported. May play a role in the regulation of lymphocyte development.

Protein type: Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: internal side of plasma membrane; cytoskeleton; cytoplasm; cytosol

Molecular Function: protein binding; protein tyrosine phosphatase activity

Biological Process: protein amino acid dephosphorylation