PPM1F active protein

PPM1F, human recombinant

Cat. Num. AAA844493

• Gene Names: PPM1F; CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: PPM1F; human recombinant; Protein phosphatase 1F; CAMKP; CaMKPase; FEM-2; hFEM-2; POPX2; PPM1F active protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: 1 mg/ml solution 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 150 mM NaCl and 1 mM DTT.; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSSGAPQ KSSPMASGAE ETPGFLDTLL QDFPALLNPE DPLPWKAPGT VLSQEEVEGE LAELAMGFLG SRKAPPPLAA ALAHEAVSQL LQTDLSEFRK LPREEEEEEE DDDEEEKAPV TLLDAQSLAQ SFFNRLWEVA GQWQKQVPLA ARASQRQWLV SIHAIRNTRR KMEDRHVSLP SFNQLFGLSD PVNRAYFAVF DGHGGVDAAR YAAVHVHTNA ARQPELPTDP EGALREAFRR TDQMFLRKAK RERLQSGTTG VCALIAGATL HVAWLGDSQV ILVQQGQVVK LMEPHRPERQ DEKARIEALG GFVSHMDCWR VNGTLAVSRA IGDVFQKPYV
• Sequence Length: 454

• Applicable Applications for PPM1F active protein: SDS-PAGE
• Biological Activity: Specific activity is > 1200 unit/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37 degree C, pH 7.5 using 10 mM of substrate.
• Results: Specific activity is > 1200 unit/mg
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for PPM1F active protein

Background: PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. Recombinant human PPM1F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family for PPM1F active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z) Cell Damage & Oxidative Stress; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 7661862
• NCBI GeneID: 9647
• NCBI Accession #: NP_055449.1
• NCBI GenBank Nucleotide #: NM_014634.3
• UniProt Accession #: P49593; Q96PM2; A8K6G3; B7Z2C3
• Molecular Weight: 52 kDa (477 aa, 1-454 aa + His Tag)
• NCBI Official Full Name: protein phosphatase 1F
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent, 1F
• NCBI Official Symbol: PPM1F
• NCBI Official Synonym Symbols: CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase
• NCBI Protein Information: protein phosphatase 1F
• UniProt Protein Name: Protein phosphatase 1F
• Protein Family: Protein phosphatase
• UniProt Gene Name: PPM1F
• UniProt Synonym Gene Names: KIAA0015; POPX2; CaM-kinase phosphatase; CaMKPase; hFem-2
• UniProt Entry Name: PPM1F_HUMAN

NCBI Description

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

PPM1F: a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members can be negative regulators of cell stress response pathways. Interact with PAK-interacting exchange factor beta (ARHGEF7), blocking the effects PAK1, a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CaMK2-gamma) is one of the substrates of this phosphatase. Promotes apoptosis.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.16; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 22q11.22

Cellular Component: protein complex; perinuclear region of cytoplasm; cytosol

Molecular Function: protein binding; calmodulin-dependent protein phosphatase activity; metal ion binding; phosphoric monoester hydrolase activity; protein serine/threonine phosphatase activity

Biological Process: histone dephosphorylation; positive regulation of focal adhesion formation; positive regulation of chemotaxis; positive regulation of stress fiber formation; negative regulation of peptidyl-serine phosphorylation; negative regulation of protein kinase activity; positive regulation of caspase activity; negative regulation of transcription, DNA-dependent; positive regulation of growth