SDS-Page
DuSP21 recombinant protein
DuSP21, 1-190aa, Human, His tag, E Coli
Gene Names
DUSP21; LMWDSP21
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
DuSP21; 1-190aa; Human; His tag; E Coli; Dual specificity protein phosphatase 21; DuSP21 recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl
Concentration
0.25mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMTASAS SFSSSQGVQQ PSIYSFSQIT RSLFLSNGVA ANDKLLLSSN RITAIVNASV EVVNVFFEGI QYIKVPVTDA RDSRLYDFFD PIADLIHTID MRQGRTLLHC MAGVSRSASL CLAYLMKYHS MSLLDAHTWT KSRRPIIRPN NGFWEQLINY EFKLFNNNTV RMINSPVGNI PDIYEKDLRM MISM
Sequence Length
190
Applicable Applications for DuSP21 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for DuSP21 recombinant protein
DuSP21 (Dual specificity protein phosphatase 21) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases (DuSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. This enzyme can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. It is exclusively expressed in testis where is preferentially dephosphorylates phosphotyrosine residues in MAPK peptides. Recombinant human DuSP21 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for DuSP21 recombinant protein
References
Hood, K.L., et al. (2002) Biochem. Biophys. Res. Commun. 298: 545-551.; Keyse, S.M. (1995) Biochim. Biophys. Acta 1265: 152-160.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24.1kDa (214aa), confirmed by MALDI-TOF
NCBI Official Full Name
dual specificity protein phosphatase 21
NCBI Official Synonym Full Names
dual specificity phosphatase 21
NCBI Official Symbol
DUSP21
NCBI Official Synonym Symbols
LMWDSP21
NCBI Protein Information
dual specificity protein phosphatase 21
UniProt Protein Name
Dual specificity protein phosphatase 21
Protein Family
UniProt Gene Name
DUSP21
UniProt Synonym Gene Names
LMWDSP21; LMW-DSP21
NCBI Description
This gene encodes a member of the dual specificity phosphatase family, specifically the low molecular weight dual specificity phosphatase family. The encoded protein localizes to both the cytoplasm and the nucleus and functions to remove phosphate groups from phosphotyrosine and phosphothreonine residues.[provided by RefSeq, Mar 2009]
Uniprot Description
Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine.
Research Articles on DuSP21
Similar Products
Product Notes
The DuSP21 dusp21 (Catalog #AAA204784) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's DuSP21 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the DuSP21 dusp21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMTASAS SFSSSQGVQQ PSIYSFSQIT RSLFLSNGVA ANDKLLLSSN RITAIVNASV EVVNVFFEGI QYIKVPVTDA RDSRLYDFFD PIADLIHTID MRQGRTLLHC MAGVSRSASL CLAYLMKYHS MSLLDAHTWT KSRRPIIRPN NGFWEQLINY EFKLFNNNTV RMINSPVGNI PDIYEKDLRM MISM. It is sometimes possible for the material contained within the vial of "DuSP21, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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