DUSP23 active protein

DUSP23, human recombinant

Cat. Num. AAA845279

• Gene Names: DUSP23; VHZ; MOSP; LDP-3; DUSP25
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: DUSP23; human recombinant; Dual specificity protein phosphatase 23; DUSP25; LDP-3; MOSP; VHZ; DUSP23 active protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 100 mM NaCl and 2 mM DTT; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGVQPPNFSW VLPGRLAGLA LPRLPAHYQF LLDLGVRHLV SLTERGPPHS DSCPGLTLHR LRIPDFCPPA PDQIDRFVQI VDEANARGEA VGVHCALGFG RTGTMLACYL VKERGLAAGD AIAEIRRLRP GSIETYEQEK AVFQFYQRTK
• Sequence Length: 150

• Applicable Applications for DUSP23 active protein: SDS-PAGE
• Biological Activity: Specific activity is > 320 unit/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37 degree C, pH7.5 using 10 mM of substrate.
• Results: Specific activity is > 320 unit/mg
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for DUSP23 active protein

Background: DuSP23, also known as low molecular mass dual specificity phosphatase 3(LDP-3), belongs to the protein-tyrosine phosphatase family. This protein is a protein phosphatase that mediates dephosphorylation of proteins phosphorylated on Tyr and Ser/Thr residues. In vitro, it can dephosphorylate p44-ERK1 (MAPK3) but not p54 SAPK-beta (MAPK10) in vitro. This protein able to enhance activation of JNK and p38(MAPK14). Recombinant human DuSP23 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family for DUSP23 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 56786144
• NCBI GeneID: 54935
• NCBI Accession #: NP_060293.2
• NCBI GenBank Nucleotide #: NM_017823.3
• UniProt Accession #: Q9BVJ7; Q9NX48
• Molecular Weight: 18.8 kDa (170 aa, 1-150 aa + His Tag)
• NCBI Official Full Name: dual specificity protein phosphatase 23
• NCBI Official Synonym Full Names: dual specificity phosphatase 23
• NCBI Official Symbol: DUSP23
• NCBI Official Synonym Symbols: VHZ; MOSP; LDP-3; DUSP25
• NCBI Protein Information: dual specificity protein phosphatase 23
• UniProt Protein Name: Dual specificity protein phosphatase 23
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP23
• UniProt Synonym Gene Names: LDP3; VHZ; LDP-3
• UniProt Entry Name: DUS23_HUMAN

Uniprot Description

DUSP23: Protein phosphatase that mediates dephosphorylation of proteins phosphorylated on Tyr and Ser/Thr residues. In vitro, it can dephosphorylate p44-ERK1 (MAPK3) but not p54 SAPK-beta (MAPK10) in vitro. Able to enhance activation of JNK and p38 (MAPK14). Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Protein type: Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis; EC 3.1.3.48; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 1q23.2

Cellular Component: cytosol; nucleus

Molecular Function: protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity