PPP1CA active protein

Human Recombinant PPP1CA

Cat. Num. AAA844825

• Gene Names: PPP1CA; PP1A; PP-1A; PPP1A; PP1alpha
• Applications: SDS-Page
• Purity: >=80%
• Synonyms: PPP1CA; Human Recombinant PPP1CA; Serine/threonine-protein phosphatase PP1-alpha catalytic subunit; PP-1A; PP1alpha; PPP1A.; PPP1CA active protein

• Host: E Coli
• Purity/Purification: >=80%
• Form/Format: 0.25 mg/ml solution in 50 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 1 mM DTT, 0.15 M NaCl, 1 mM MnCl2 and 0.1 mM PMSF.; Appearance: Liquid
• Concentration: 0.25 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK
• Sequence Length: 341

• Applicable Applications for PPP1CA active protein: SDS-PAGE
• Biological Activity: Specific activity: >3,000 units/mg.
• Results: >3,000 units/mg
• Unit Definition: One unit will hydrolyze 1 nmole of p-nitrophenylphosphatate (pNPP) per minute at 37 degree C, pH 7.5 using 10 mM of substrate.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for PPP1CA active protein

Background: Serine/Threonine-protein phosphatase PP1-alpha catalytic subunit (PPP1CA) is crucial for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. The PPP1CA protein may have a significant role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.

Product Categories/Family for PPP1CA active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 56790945
• NCBI GeneID: 5499
• NCBI Accession #: NP_001008709.1
• NCBI GenBank Nucleotide #: NM_001008709.1
• UniProt Accession #: P62136; P08129; P20653; P22802; Q07161; A6NNR3; B2R908
• Molecular Weight: 39.7 kDa (350 aa, 1-330 aa + NT His-Tag)
• NCBI Official Full Name: serine/threonine-protein phosphatase PP1-alpha catalytic subunit isoform 3
• NCBI Official Synonym Full Names: protein phosphatase 1, catalytic subunit, alpha isozyme
• NCBI Official Symbol: PPP1CA
• NCBI Official Synonym Symbols: PP1A; PP-1A; PPP1A; PP1alpha
• NCBI Protein Information: serine/threonine-protein phosphatase PP1-alpha catalytic subunit
• UniProt Protein Name: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP1CA
• UniProt Synonym Gene Names: PPP1A; PP-1A
• UniProt Entry Name: PP1A_HUMAN

NCBI Description

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PPP1CA: is a catalytic subunit of protein phosphatase 1 (PP1), aubiquitous cytosolic serine-threonine phosphatase. Composed of a catalytic subunit (PPP1CA, PPP1CB or PPP1CC) which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting (or regulatory) subunits: PPP1R12A and PPP1R12B mediate binding to myosin; PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen; and PPP1R15A and PPP1R15B mediate binding to EIF2S1. PP1 is essential for cell division, plays critical roles in many cellular processes including glycogen metabolism, muscle contractility and protein synthesis, and is involved in the regulation of ionic conductances and long-term synaptic plasticity. Inhibits the synthesis of proteins involved in synaptic plasticity. Binds to the transcription factor cyclic AMP-dependent response element binding (CREB) and dephosphorylates it. May regulate chromatin condensation through regulation of histone H3 phosphorylation. Differentially expressed in gastric cancer. May participate in the neurodegenerative progress in Alzheimer's disease. Down-regulated in lung squamous cell carcinoma.

Protein type: Nucleolus; EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; glycogen granule; cytoplasm; nucleolus; dendritic spine; plasma membrane; perikaryon; protein phosphatase type 1 complex; cytosol; nucleus

Molecular Function: ribonucleoprotein binding; protein binding; metal ion binding; phosphoric monoester hydrolase activity; protein serine/threonine phosphatase activity

Biological Process: glycogen metabolic process; regulation of glycogen biosynthetic process; regulation of circadian rhythm; cell cycle; protein amino acid dephosphorylation; entrainment of circadian clock by photoperiod; regulation of glycogen catabolic process; branching morphogenesis of a tube; cell division; transforming growth factor beta receptor signaling pathway; triacylglycerol catabolic process; circadian regulation of gene expression; negative regulation of transforming growth factor beta receptor signaling pathway; lung development