Testing Data
NME3 active protein
NME3, human recombinant
Applications
SDS-Page
Purity
>=95%
Synonyms
NME3; human recombinant; Nucleoside diphosphate kinase 3; c371H6.2; DR-nm23; KIAA0516; NDPK-C; NDPKC; NM23-H3; NM23H3.; NME3 active protein
Host
E Coli
Purity/Purification
>=95%
Form/Format
0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.1 M NaCl and 2 mM DTT
Appearance: Liquid
Appearance: Liquid
Concentration
0.5 mg/ml (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV ESARREIALW FRADELLCWE DSAGHWLYE
Applicable Applications for NME3 active protein
SDS-PAGE
Biological Activity
Specific activity is > 20 units/ml, in which one unit will convert 1.0 umole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25 degree C in a coupled system with PK/LDH.
Results
Specific activity is > 20 units/ml
Unit Definition
One unit will convert 1.0 umole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25 degree C in a coupled system with PK/LDH.
Handling
Centrifuge the vial prior to opening.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for NME3 active protein
Background: NME3, also known as, a potential suppressor of metastasis, is expressed at a much lower level in highly metastatic cells than in cells with lower metastatic potential. It is important for the synthesis of nucleoside triphosphates and may play a role in apoptosis induction and hematopoiesis. It is preferentially expressed during early stages of myeloid differentiation of highly purified CD34+ cells. Recombinant human NME3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Product Categories/Family for NME3 active protein
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
19.1 kDa (169 aa, 22-169 aa + His Tag)
Protein Family
Similar Products
Product Notes
The NME3 (Catalog #AAA844811) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NME3 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the NME3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV ESARREIALW FRADELLCWE DSAGHWLYE. It is sometimes possible for the material contained within the vial of "NME3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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