CD3/CD4 Dual Color Reagent | CD3 dual color reagent
ANTI DOG CD3:FITC/CD4:RPE
Applications
Flow cytometry
Synonyms
CD3/CD4; ANTI DOG CD3:FITC/CD4:RPE; CD3 dual color reagent
Isotype
Cocktail
FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG2a (RAT)
FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG2a (RAT)
Clone Number
CA17.2A12/YKIX302.9
Form/Format
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized.
Sequence Length
202
Applicable Applications for CD3 dual color reagent
Flow cytometry
Application Notes
Dilution: Neat
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Reconstitution
Reconstitute with 1.0 ml distilled water
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
1% Bovine Serum Albumin
5% Sucrose
Max Ex/Em
Fluorophore: FITC / Excitation Max: 490 (nm) / Emission Max (nm): 525
Fluorophore: RPE 488nm laser / Excitation Max: 496 (nm) / Emission Max (nm): 578
Fluorophore:RPE 561nm laser / Excitation Max: 546 (nm) / Emission Max (nm): 578
Fluorophore: RPE 488nm laser / Excitation Max: 496 (nm) / Emission Max (nm): 578
Fluorophore:RPE 561nm laser / Excitation Max: 546 (nm) / Emission Max (nm): 578
Buffer Solution
Phosphate buffered saline
Target Species
Dog
Preparation and Storage
Prior to reconstitution store at 4°C. Following reconstitution store at 4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Related Product Information for CD3 dual color reagent
MBS238972 is a dual colour reagent recognizing canine CD3 and CD4 cell surface antigens. Clone CA17.2A12 recognizes canine CD3, a T cell differentiation antigen expressed on mature T lymphocytes.
Clone YKIX 302.9 recognizes canine CD4, which is expressed by lymphocyte subsets and canine neutrophils.
Clone YKIX 302.9 recognizes canine CD4, which is expressed by lymphocyte subsets and canine neutrophils.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
T-cell surface glycoprotein CD3 epsilon chain
NCBI Official Symbol
CD3E
NCBI Protein Information
T-cell surface glycoprotein CD3 epsilon chain; CD3E antigen, epsilon polypeptide (TiT3 complex)
UniProt Protein Name
T-cell surface glycoprotein CD3 epsilon chain
UniProt Gene Name
CD3E
UniProt Entry Name
CD3E_CANFA
Uniprot Description
The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses.
Research Articles on CD3
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Product Notes
The CD3 cd3e (Catalog #AAA238972) is a Dual Color Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD3/CD4 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry. Dilution: Neat Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Researchers should empirically determine the suitability of the CD3 cd3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3/CD4, Dual Color Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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