MPO/CD3 Dual Color Reagent | CD3G dual color reagent

MOUSE ANTI HUMAN MYELOPEROXIDASE:FITC/CD3:RPE

Cat. Num. AAA238078

• Gene Names: CD3G; T3G; IMD17; CD3-GAMMA
• Applications: Flow Cytometry, Functional Assay
• Synonyms: MPO/CD3; MOUSE ANTI HUMAN MYELOPEROXIDASE:FITC/CD3:RPE; CD3G dual color reagent

• Host: Mouse
• Isotype: FITC reagent: IgG1 (MOUSE) ; RPE reagent: IgG1 (MOUSE)
• Clone Number: 2C7/UCHT1
• Specificity: UCHT-1 recognises the human CD3 antigen, which is expressed by all mature T cells at the cell surface. CD3 is expressed in the cytoplasm of immature T cells.2C7 recognises myeloperoxidase, an intracellular enzyme expressed by cells of myeloid lineage.
• Form/Format: FITC/RPE; Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
• Sequence Length: 182

• Applicable Applications for CD3G dual color reagent: Flow cytometry (FC/FACS)*
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood. ; Flow Cytometry: Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
• Reconstitution: Reconstitute with 0.5 ml distilled water; Perservative Stabilisers
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(MBS238078 staining of permeabilised human peripheral blood leucocytes)

Related Product Information for CD3G dual color reagent

UCHT-1 recognises the human CD3 antigen, which is expressed by all mature T cells at the cell surface. CD3 is expressed in the cytoplasm of immature T cells. 2C7 recognises myeloperoxidase, an intracellular enzyme expressed by cells of myeloid lineage.

NCBI and Uniprot Product Information

• NCBI GI #: 4557429
• NCBI GeneID: 917
• NCBI Accession #: NP_000064.1
• NCBI GenBank Nucleotide #: NM_000073.2
• Molecular Weight: 20,469 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 gamma chain
• NCBI Official Synonym Full Names: CD3g molecule, gamma (CD3-TCR complex)
• NCBI Official Symbol: CD3G
• NCBI Official Synonym Symbols: T3G; IMD17; CD3-GAMMA
• NCBI Protein Information: T-cell surface glycoprotein CD3 gamma chain; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); T-cell antigen receptor complex, gamma subunit of T3; T-cell receptor T3 gamma chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 gamma chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD3G
• UniProt Synonym Gene Names: T3G
• UniProt Entry Name: CD3G_HUMAN

NCBI Description

The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

CD3G: The CD3 complex mediates signal transduction.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; alpha-beta T cell receptor complex

Molecular Function: receptor signaling complex scaffold activity; transmembrane receptor activity; protein heterodimerization activity; T cell receptor binding

Biological Process: regulation of immune response; protein transport; cell surface receptor linked signal transduction; T cell activation; T cell costimulation; establishment and/or maintenance of cell polarity; innate immune response; protein complex assembly; T cell receptor signaling pathway

Disease: Immunodeficiency 17