CD3/CD45RA Dual Color Reagent | CD3 dual color reagent

MOUSE ANTI RAT CD3:FITC/CD45RA:RPE

Cat. Num. AAA238087

• Gene Names: Ptprc; Lca; RT7; CD45; L-CA; T200
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD3/CD45RA; MOUSE ANTI RAT CD3:FITC/CD45RA:RPE; CD3 dual color reagent

• Host: Mouse
• Isotype: FITC reagent: IgM (MOUSE) ; RPE reagent: IgG1 (MOUSE)
• Clone Number: IF4/OX-33
• Specificity: This item is a dual colour reagent recognising rat CD3 and CD45RA cell surface antigens. ; ; Clone IF4 recognises rat CD3, a cell surface antigen expressed by mature lymphocytes. ; ; Clone OX-33 recognises rat CD45RA, a cell surface antigen expressed by B-lymphocytes.
• Form/Format: FITC/RPE; Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
• Sequence Length: 1190

• Applicable Applications for CD3 dual color reagent: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cell in 100ul. ; Flow Cytometry: Maximum Dilution: Neat
• Reconstitution: Reconstitute with 1 ml distilled water; Perservative Stabilisers
• Buffer Solution: Phosphate buffered saline
• Target Species: Rat
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Staining of rat spleen cells with Mouse anti Rat CD3:FITC/CD45RA:RPE (MBS238087))

Related Product Information for CD3 dual color reagent

MBS238087 is a dual colour reagent recognising rat CD3 and CD45RA cell surface antigens. Clone IF4 recognises rat CD3, a cell surface antigen expressed by mature lymphocytes. Clone OX-33 recognises rat CD45RA, a cell surface antigen expressed by B-lymphocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 158138490
• NCBI GeneID: 24699
• NCBI Accession #: NP_001103357.1
• NCBI GenBank Nucleotide #: NM_001109887.1
• Molecular Weight: 134,182 Da
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase C isoform 3
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, C
• NCBI Official Symbol: Ptprc
• NCBI Official Synonym Symbols: Lca; RT7; CD45; L-CA; T200
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase C; Protein tyrosine phosphatase, receptor-type, c polypeptide; T200 glycoprotein; leucocyte common antigen; leukocyte common antigen A; leukocyte common antigen B
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase C
• UniProt Gene Name: Ptprc
• UniProt Synonym Gene Names: L-CA
• UniProt Entry Name: PTPRC_RAT

NCBI Description

member of a family of heavily glycosylated leukocyte cell surface glycoproteins; displays extensive O-glycosylation [RGD, Feb 2006]

Uniprot Description

CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described.

Protein type: EC 3.1.3.48; Membrane protein, integral; Receptor protein phosphatase, tyrosine; Motility/polarity/chemotaxis

Cellular Component: internal side of plasma membrane; cell surface; focal adhesion; membrane; integral to plasma membrane; integral to membrane; plasma membrane; intracellular; cytosol; external side of plasma membrane; lipid raft

Molecular Function: heparin binding; heparan sulfate proteoglycan binding; protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein kinase regulator activity; protein kinase binding

Biological Process: B cell proliferation; positive regulation of isotype switching to IgG isotypes; activation of MAPK activity; regulation of humoral immune response mediated by circulating immunoglobulin; regulation of cell cycle; positive regulation of T cell mediated cytotoxicity; protein amino acid dephosphorylation; T cell receptor signaling pathway; T cell proliferation; leukocyte adhesion; B cell receptor signaling pathway; positive regulation of MAPKKK cascade; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; positive regulation of T cell mediated immunity; response to gamma radiation; positive regulation of T cell proliferation; negative regulation of protein amino acid autophosphorylation; negative regulation of cytokine and chemokine mediated signaling pathway; positive regulation of gamma-delta T cell differentiation; defense response to virus; T cell differentiation; positive thymic T cell selection; regulation of B cell receptor signaling pathway; negative regulation of peptidyl-tyrosine phosphorylation; positive regulation of humoral immune response mediated by circulating immunoglobulin; substrate-bound cell migration, cell release from substrate; negative thymic T cell selection; positive regulation of antigen receptor-mediated signaling pathway; bone marrow development; heterotypic cell-cell adhesion; stem cell development; positive regulation of T cell differentiation; dephosphorylation; B cell differentiation; release of sequestered calcium ion into cytosol; regulation of B cell differentiation; negative regulation of protein kinase activity; positive regulation of alpha-beta T cell proliferation; hemopoietic progenitor cell differentiation; immunoglobulin biosynthetic process