Testing Data
(Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD21:RPE)
Mouse CD21 Monoclonal Antibody | anti-CD21 antibody
MOUSE ANTI CANINE CD21:RPE
Reactivity
Horse, Cat, RacoonN.B. Antibody reactivity and working conditions vary between species
Applications
Flow cytometry
Synonyms
CD21; Monoclonal Antibody; MOUSE ANTI CANINE CD21:RPE; anti-CD21 antibody
Host
Mouse
Reactivity
Horse, Cat, Racoon
N.B. Antibody reactivity and working conditions vary between species
N.B. Antibody reactivity and working conditions vary between species
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CA2.1D6
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Applicable Applications for anti-CD21 antibody
Flow cytometry
Application Notes
Dilution: Neat
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
Note:N.B. It should NOT be used with (mouse anti canine CD3), in dual colour flow cytometry, due to non-specific interactions between the two reagents.
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood.
Note:N.B. It should NOT be used with (mouse anti canine CD3), in dual colour flow cytometry, due to non-specific interactions between the two reagents.
Reconstitution
Reconstitute with 1 ml distilled water
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
1% Bovine Serum Albumin
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Max Ex/Em
Fluorophore
PE 488nm laser
PE 561nm laser
Excitation (nm):
496
546
Emission (nm):
578
578
PE 488nm laser
PE 561nm laser
Excitation (nm):
496
546
Emission (nm):
578
578
Buffer Solution
Phosphate buffered saline
Target Species
Dog
Preparation and Storage
Prior to reconstitution store at 4°C. Following reconstitution store at 4°C.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Testing Data
(Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD21:RPE)
Testing Data
(Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD21:RPE)
Testing Data
(Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD21:Alexa Fluor 647 )
Testing Data
(Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD21:Alexa Fluor 647 )
Testing Data
(Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD21 (MBS215220))
Testing Data
(Staining of canine peripheral blood lymphocytes with Mouse anti Canine CD21 (MBS215220))
Related Product Information for anti-CD21 antibody
Mouse anti Canine CD21 antibody, clone CA2.1D6 recognizes canine CD21, also known as Complement receptor type 2. CD21is a cell surface antigen expressed by canine B lymphocytes.
The antigen recognised may be the canine homologue of human CD21, but this has not been fully confirmed.
Mouse anti Canine CD21 antibody, clone CA2.1D6 also recognizes the CD21 antigen in Felids. Expression in cats is analogous to that seen in dogs with strong expression on lymphocytes, in a manner mutually exclusive with expression of CD4 or CD8. Clone CA2.1D6 also immunoprecipitates a 145kDa protein from feline lymphocytes, similar to the protein immunoprecipitated by the antibody from canine lymphocytes (Dean et al. 1996).
The antigen recognised may be the canine homologue of human CD21, but this has not been fully confirmed.
Mouse anti Canine CD21 antibody, clone CA2.1D6 also recognizes the CD21 antigen in Felids. Expression in cats is analogous to that seen in dogs with strong expression on lymphocytes, in a manner mutually exclusive with expression of CD4 or CD8. Clone CA2.1D6 also immunoprecipitates a 145kDa protein from feline lymphocytes, similar to the protein immunoprecipitated by the antibody from canine lymphocytes (Dean et al. 1996).
Similar Products
Product Notes
The CD21 (Catalog #AAA215219) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI CANINE CD21:RPE reacts with Horse, Cat, Racoon N.B. Antibody reactivity and working conditions vary between species and may cross-react with other species as described in the data sheet. AAA Biotech's CD21 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry. Dilution: Neat Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood. Note:N.B. It should NOT be used with (mouse anti canine CD3), in dual colour flow cytometry, due to non-specific interactions between the two reagents. Researchers should empirically determine the suitability of the CD21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD21, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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