Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse anti Pig CD4 . All experiments performed on frozen porcine blood gated on live single cell lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer)
Mouse anti-Pig CD4 Monoclonal Antibody | anti-CD4 antibody
Mouse Anti Pig CD4: Alexa Fluor 647
Reactivity
Pig
Applications
Flow Cytometry
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Synonyms
CD4; Monoclonal Antibody; Mouse Anti Pig CD4: Alexa Fluor 647; CD4 antibody (b38c6c7); Mouse anti Pig CD4; anti-CD4 antibody
Host
Mouse
Reactivity
Pig
Clonality
Monoclonal
Isotype
IgG1
Clone Number
b38c6c7
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Form/Format
Liquid; Phosphate Buffered Saline
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
487
Applicable Applications for anti-CD4 antibody
Flow Cytometry (FC)
Application Notes
Suggested Dilution- Min: Neat/Max: 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunogen
Porcine leukocytes
Conjugation
ALEXA FLUOR 647
Approx. Protein Concentrations
IgG concentration 0.05 mg/mL
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
1% Bovine Serum Albumin
Max Ex/Em
Fluorophore: Alexa FluorR647
Excitation Max (nm): 650
Emission Max (nm): 665
Excitation Max (nm): 650
Emission Max (nm): 665
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse anti Pig CD4 . All experiments performed on frozen porcine blood gated on live single cell lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse anti Pig CD4 . All experiments performed on frozen porcine blood gated on live single cell lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD5 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control detected with Goat anti Mouse IgG1:RPE . Figure B. FITC conjugated Mouse anti Pig CD5 and Mouse anti Pig CD4 detected with Goat anti Mouse IgG1:RPE . All experiments performed on frozen porcine blood gated on live single cell lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD5 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control detected with Goat anti Mouse IgG1:RPE . Figure B. FITC conjugated Mouse anti Pig CD5 and Mouse anti Pig CD4 detected with Goat anti Mouse IgG1:RPE . All experiments performed on frozen porcine blood gated on live single cell lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. RPE conjugated Mouse anti Pig CD3 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Pig CD3 and FITC conjugated Mouse anti Pig CD4 . All experiments performed on frozen porcine blood gated on live single cell lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. RPE conjugated Mouse anti Pig CD3 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Mouse anti Pig CD3 and FITC conjugated Mouse anti Pig CD4 . All experiments performed on frozen porcine blood gated on live single cell lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)
Related Product Information for anti-CD4 antibody
Mouse anti Porcine CD4, clone b38c6c7 recognizes a ~55 kDa porcine homologue of the human CD4 antigen found on the surface of T helper cells. Clone b38c6c7 was confirmed to bind to a member of the CD4 alpha cluster at the 'Third International Workshop on Swine Leukocyte Differentiation Antigens' (Haverson et al. 2001).
Porcine CD4 is a type 1 transmembrane member of the immunoglobulin superfamily. Pigs are unusual among mammalian species as they appear to have four populations of resting T lymphocytes. In addition to CD4+/CD8- and CD4-/CD8+ lymphocytes they also appear to have significant populations of CD4-/CD8- and CD4+/CD8+ cells. Lymphoblasts with a double positive phenotype have been described in other species but not in the case of mature peripheral T cells (Saalmuller et al. 1987).
Porcine CD4 is a type 1 transmembrane member of the immunoglobulin superfamily. Pigs are unusual among mammalian species as they appear to have four populations of resting T lymphocytes. In addition to CD4+/CD8- and CD4-/CD8+ lymphocytes they also appear to have significant populations of CD4-/CD8- and CD4+/CD8+ cells. Lymphoblasts with a double positive phenotype have been described in other species but not in the case of mature peripheral T cells (Saalmuller et al. 1987).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
CD4
NCBI Official Synonym Full Names
CD4 molecule
NCBI Official Symbol
CD4
NCBI Protein Information
T-cell surface glycoprotein CD4
UniProt Protein Name
CD4
Protein Family
UniProt Gene Name
CD4
Research Articles on CD4
Similar Products
Product Notes
The CD4 cd4 (Catalog #AAA225684) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Pig CD4: Alexa Fluor 647 reacts with Pig and may cross-react with other species as described in the data sheet. AAA Biotech's CD4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC). Suggested Dilution- Min: Neat/Max: 1/5 Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the CD4 cd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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