SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Disulfide oxidoreductase (DsbA) Recombinant Protein | DsbA recombinant protein
Disulfide oxidoreductase (DsbA), 20-208 aa, E Coli, E Coli
Gene Names
dsbA; dsf; ECK3852; iarA; JW3832; ppfA
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
Disulfide oxidoreductase (DsbA); 20-208 aa; E Coli; Disulfide oxidoreductase A; dsbA; rpbB; Disulfide oxidoreductase (DsbA) E Coli; DsDNA-binding protein A; Double-stranded DNA-binding protein; Disulfide oxidoreductase A periplasmic protein disulfide isomerase I; Thiol disulfide interchange protein dsbA.; DsbA recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. 20mM Tris pH 7.5, 2mM EDTA
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MAQYEDGKQY TTLEKPVAGA PQVLEFFSFF CPHCYQFEEV LHISDNVKKK LPEGVKMTKY HVNFMGGDLG KDLTQAWAVA MALGVEDKVT VPLFEGVQKT QTIRSASDIR DVFINAGIKG EEYDAAWNSF VVKSLVAQQE KAAADVQLRG VPAMFVNGKY QLNPQGMDTS NMDVFVQQYA DTVKYLSEKK
Sequence Length
208
Applicable Applications for DsbA recombinant protein
SDS-PAGE
Domain
20-208aa
Tag
Non-Tagged
Preparation and Storage
Can be stored at 2°C to 8°C for one week.
For long term storage, aliquot and store at -20°C or -80°C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20°C or -80°C.
Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for DsbA recombinant protein
Description: Recombinant Disulfide Oxidoreductase (rDsbA), produced from E. coli is a periplasmic protein and thioredoxin superfamily member which introduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active-site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA consists of 208 amino acids containing signal peptide (1-19 amino acids). Recombinant DsbA (residues 20-208) was expressed.
Product Categories/Family for DsbA recombinant protein
References
Charbonnier, J. B. et al. (1999). Protein Sci. 8:96-105; Inaba, K. et al. (2004). J. Biol. Chem. 279:6761-6768.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21.2kDa (190aa), confirmed by MALDI-TOF.
NCBI Official Full Name
periplasmic protein disulfide isomerase I
NCBI Official Symbol
dsbA
NCBI Official Synonym Symbols
dsf; ECK3852; iarA; JW3832; ppfA
NCBI Protein Information
periplasmic protein disulfide isomerase I
UniProt Protein Name
Thiol:disulfide interchange protein DsbA
Protein Family
UniProt Gene Name
dsbA
UniProt Synonym Gene Names
dsf; ppfA
NCBI Description
DsbA has a role in cytochrome C synthesis. [More information is available at EcoGene: EG11297].
Uniprot Description
Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.
Research Articles on DsbA
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Product Notes
The DsbA dsba (Catalog #AAA203035) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Disulfide oxidoreductase (DsbA) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the DsbA dsba for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MAQYEDGKQY TTLEKPVAGA PQVLEFFSFF CPHCYQFEEV LHISDNVKKK LPEGVKMTKY HVNFMGGDLG KDLTQAWAVA MALGVEDKVT VPLFEGVQKT QTIRSASDIR DVFINAGIKG EEYDAAWNSF VVKSLVAQQE KAAADVQLRG VPAMFVNGKY QLNPQGMDTS NMDVFVQQYA DTVKYLSEKK. It is sometimes possible for the material contained within the vial of "Disulfide oxidoreductase (DsbA), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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