Western Blot (WB)
(Western blot detection of DsbA with Dsba recombinant protein using DsbA mouse mAb (1:1000 diluted).Predicted band size: 24KDa.Observed band size: 24KDa.)
Mouse DsbA-Tag Monoclonal Antibody | anti-dsbA antibody
DsbA-Tag Antibody
Gene Names
dsbA; dsf; ECK3852; iarA; JW3832; ppfA
Applications
Western Blot
Synonyms
DsbA-Tag; Monoclonal Antibody; DsbA-Tag Antibody; Purified Mouse Monoclonal Antibody (Mab); DSBA like thioredoxin domain containing protein; anti-dsbA antibody
Host
Mouse
Clonality
Monoclonal
Form/Format
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.
Sequence Length
208
Applicable Applications for anti-dsbA antibody
Western Blot (WB)
Application Notes
WB ~~ 1:1000
Antigen Source
E Coli
Antigen Type
Recombinant Protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot detection of DsbA with Dsba recombinant protein using DsbA mouse mAb (1:1000 diluted).Predicted band size: 24KDa.Observed band size: 24KDa.)
Western Blot (WB)
(Western blot detection of DsbA with Dsba recombinant protein using DsbA mouse mAb (1:1000 diluted).Predicted band size: 24KDa.Observed band size: 24KDa.)
Related Product Information for anti-dsbA antibody
Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.
Product Categories/Family for anti-dsbA antibody
References
Bardwell J.C.A.,et al.Cell 67:581-589(1991). Kamitani S.,et al.EMBO J. 11:57-62(1992). Plunkett G. III,et al.Nucleic Acids Res. 21:3391-3398(1993). Blattner F.R.,et al.Science 277:1453-1462(1997). Hayashi K.,et al.Mol. Syst. Biol. 2:E1-E5(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23105
NCBI Official Full Name
periplasmic protein disulfide isomerase I
NCBI Official Symbol
dsbA
NCBI Official Synonym Symbols
dsf; ECK3852; iarA; JW3832; ppfA
NCBI Protein Information
periplasmic protein disulfide isomerase I
UniProt Protein Name
Thiol:disulfide interchange protein DsbA
Protein Family
UniProt Gene Name
dsbA
UniProt Synonym Gene Names
dsf; ppfA
UniProt Entry Name
DSBA_ECOLI
NCBI Description
DsbA has a role in cytochrome C synthesis. [More information is available at EcoGene: EG11297].
Uniprot Description
Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.
Research Articles on dsbA
Similar Products
Product Notes
The dsbA dsba (Catalog #AAA9215545) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DsbA-Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~ 1:1000. Researchers should empirically determine the suitability of the dsbA dsba for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DsbA-Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
