Ac-DEVD-AMC, inhibitor

Ac-DEVD-AMC

Cat. Num. AAA5755783

• Purity: 98.00%
• Synonyms: Ac-DEVD-AMC; inhibitor

• Purity/Purification: 98.00%
• Form/Format: Solid. Powder

• Solubility: H2O: <0.1mg/mL (insoluble); (<1mg/ml refers to the product slightly soluble or insoluble)
• Formula: C30H37N5O13
• SMILES: CC(C1=CC=C(NC([C@H](CC(O)=O)NC([C@H](C(C)C)NC([C@H](CCC(O)=O)NC([C@@H](NC(C)=O)CC(O)=O)=O)=O)=O)=O)C=C1O2)=CC2=O
• CAS Number: 169332-61-0
• Target & IC50: Others
• Cell Research: Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37 degree C in 50mL KH buffer or MEM (continuously gass with 95% O2: 5% CO2) for up to 6 hours. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32uM zVAD-fmk or 15 uL DMSO for 20 min (f/v=1.0mL). Ac-DEVD-AMC (37 uM) is then added and the incubation continues for an additional 20 minutes. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min, and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through a microcentrifuge filter, separated on HPLC, and analyzed for the fluorogenic AMC moiety [2].
• Preparation and Storage: Store at -20 degree C for 3 years powder; -80 degree C for 2 years in solvent

Structure

Related Product Information for Ac-DEVD-AMC, inhibitor

Ac-DEVD-AMC is the Caspase-3 substrate.

References

Ahmed R Alsuwaidi, et al. Bioenergetics of murine lungs infected with respiratory syncytial virus. Virol J. 2013; 10: 22.
Ahmed R Alsuwaidi, et al. Bioenergetics of murine lungs infected with respiratory syncytial virus. Virol J. 2013; 10: 22.
Ahmed R Alsuwaidi, et al. Lung tissue bioenergetics and caspase activity in rodents. BMC Res Notes. 2013; 6: 12.
Ahmed R Alsuwaidi, et al. Lung tissue bioenergetics and caspase activity in rodents. BMC Res Notes. 2013; 6: 12.