SDS-Page
(3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.)
GroEL recombinant protein
GroEL, 1-548aa, E Coli, E Coli
Gene Names
groL; ECK4137; groEL; JW4103; mopA
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
GroEL; 1-548aa; E Coli; groL; groEL; mopA; Protein Cpn60; 60 kDa chaperonin; groEL protein; chaperonin groel; GroEL recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 25 mM Tris-HCl (pH 7.5) 100mM NaCl, 5mM DTT, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MAAKDVKFGN DARVKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPTIT KDGVSVAREI ELEDKFENMG AQMVKEVASK ANDAAGDGTT TATVLAQAII TEGLKAVAAG MNPMDLKRGI DKAVTAAVEE LKALSVPCSD SKAIAQVGTI SANSDETVGK LIAEAMDKVG KEGVITVEDG TGLQDELDVV EGMQFDRGYL SPYFINKPET GAVELESPFI LLADKKISNI REMLPVLEAV AKAGKPLLII AEDVEGEALA TLVVNTMRGI VKVAAVKAPG FGDRRKAMLQ DIATLTGGTV ISEEIGMELE KATLEDLGQA KRVVINKDTT TIIDGVGEEA AIQGRVAQIR QQIEEATSDY DREKLQERVA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALI RVASKLADLR GQNEDQNVGI KVALRAMEAP LRQIVLNCGE EPSVVANTVK GGDGNYGYNA ATEEYGNMID MGILDPTKVT RSALQYAASV AGLMITTECM VTDLPKNDAA DLGAAGGMGG MGGMGGMM
Sequence Length
1-548
Applicable Applications for GroEL recombinant protein
SDS-PAGE
Antigen Species
E Coli
Preparation and Storage
Can be stored at 2-8°C for 1 week. For long term storage, aliquot and store at 20°C to -80°C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.)
SDS-Page
(3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.)
Related Product Information for GroEL recombinant protein
GroEL protein is the major heat shock protein of E Coli and belongs to the chaperonin (HSP60) family. GroEL protein prevents misfolding of proteins and promotes the refolding and proper assembly of unfolded polypeptiedes generated under stress condition. GroEL gene was amplified by PCR from E Coli and cloned into an expression vector. This protein was overexpressed in E Coli and was purified by using conventional chromatography techniques.
Product Categories/Family for GroEL recombinant protein
References
Hemmingsen,S.M., et al (1988) Nature. 333 (6171) 330-334; Braig,K., et al (1994) Nature 371 (6498) 578-586; Chen,L and Singler,P.B. (1999) Cell 99 (7) 757-768;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57.3kDa (548aa), confirmed by MALDI-TOF.
NCBI Official Full Name
Cpn60 chaperonin GroEL, large subunit of GroESL
NCBI Official Symbol
groL
NCBI Official Synonym Symbols
ECK4137; groEL; JW4103; mopA
NCBI Protein Information
Cpn60 chaperonin GroEL, large subunit of GroESL
UniProt Protein Name
60 kDa chaperonin
UniProt Gene Name
groL
NCBI Description
groL is an essential gene. [More information is available at EcoGene: EG10599]. The Escherichia coli chaperone protein GroEL (Hsp60) and its regulator GroES are necessary for the proper folding of certain proteins , . [More information is available at EcoCyc: EG10599].
Uniprot Description
Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.
Research Articles on GroEL
Similar Products
Product Notes
The GroEL grol (Catalog #AAA203023) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GroEL can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the GroEL grol for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MAAKDVKFGN DARVKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPTIT KDGVSVAREI ELEDKFENMG AQMVKEVASK ANDAAGDGTT TATVLAQAII TEGLKAVAAG MNPMDLKRGI DKAVTAAVEE LKALSVPCSD SKAIAQVGTI SANSDETVGK LIAEAMDKVG KEGVITVEDG TGLQDELDVV EGMQFDRGYL SPYFINKPET GAVELESPFI LLADKKISNI REMLPVLEAV AKAGKPLLII AEDVEGEALA TLVVNTMRGI VKVAAVKAPG FGDRRKAMLQ DIATLTGGTV ISEEIGMELE KATLEDLGQA KRVVINKDTT TIIDGVGEEA AIQGRVAQIR QQIEEATSDY DREKLQERVA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALI RVASKLADLR GQNEDQNVGI KVALRAMEAP LRQIVLNCGE EPSVVANTVK GGDGNYGYNA ATEEYGNMID MGILDPTKVT RSALQYAASV AGLMITTECM VTDLPKNDAA DLGAAGGMGG MGGMGGMM. It is sometimes possible for the material contained within the vial of "GroEL, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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