CYTL1 recombinant protein

CYTL1 Protein, Human, Recombinant (mFc Tag)

Cat. Num. AAA8121489

• Gene Names: CYTL1; C17; C4orf4
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CYTL1; CYTL1 Protein; Human; Recombinant (mFc Tag); Human CYTL1/Cytokine-like 1 Protein (Fc Tag); C17 Protein; C4orf4 Protein; cytokine-like 1; CYTL1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg136

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Thr 23
• Tag: C-mouse IgG1-Fc
• Protein Construction: A DNA sequence encoding the human CYTL1 (NP_061129.1)(Met1-Arg136) was expressed with the Fc region of mouse IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CYTL1 recombinant protein

Background: Cytokine-like protein 1 (CYTL1) is a small widely expressed secreted protein lacking significant primary sequence homology to any other known protein. Cytokine-like 1 (CYTL1) is a novel potential cytokine that was first identified in CD34(+) cells derived from bone marrow and cord blood, and it was also found using our immunogenomics strategy. CYTL1 expression appears to be highest in the hematopoietic system and in chondrocytes; however, maintenance of cartilage in mouse models of arthritis is its only reported function in vivo. Despite lacking sequence homology to chemokines, CYTL1is predicted by computational methods to fold like a chemokine, and has been reported to function as a chemotactic agonist at the chemokine receptor CCR2 in mouse monocyte/macrophages. Recombinant CYTL1 promoted calcium flux in chondrocytes. Cytokine-like protein 1 (Cytl1), had been described as a protein expressed in CD34+ cells, and as a functional secreted protein involved in chondrogenesis and cartilage development.

NCBI and Uniprot Product Information

• NCBI GI #: 8922108
• NCBI GeneID: 54360
• NCBI Accession #: NP_061129.1
• NCBI GenBank Nucleotide #: NM_018659.2
• UniProt Accession #: Q9NRR1
• Molecular Weight: 15,577 Da
• NCBI Official Full Name: cytokine-like protein 1
• NCBI Official Synonym Full Names: cytokine-like 1
• NCBI Official Symbol: CYTL1
• NCBI Official Synonym Symbols: C17; C4orf4
• NCBI Protein Information: cytokine-like protein 1; cytokine-like protein C17
• UniProt Protein Name: Cytokine-like protein 1
• Protein Family: Cytokine-like protein
• UniProt Gene Name: CYTL1
• UniProt Synonym Gene Names: C4orf4
• UniProt Entry Name: CYTL1_HUMAN

NCBI Description

C17 is a cytokine-like protein specifically expressed in bone marrow and cord blood mononuclear cells that bear the CD34 (MIM 142230) surface marker (Liu et al., 2000 [PubMed 10857752]).[supplied by OMIM, Mar 2008]

Uniprot Description

CYTL1:

Protein type: Cell development/differentiation; Transcription factor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4p16-p15

Cellular Component: extracellular space

Molecular Function: receptor binding

Biological Process: chondroitin sulfate proteoglycan biosynthetic process; chondrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; signal transduction; inner ear development