FSTL1 recombinant protein

FSTL1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120582

• Gene Names: FSTL1; FRP; FSL1; MIR198
• Purity: >98% as determined by SDS-PAGE
• Synonyms: FSTL1; FSTL1 Protein; Human; Recombinant (His Tag); Human FSTL1 Protein (His Tag); FRP Protein; FSL1 Protein; MIR198 Protein; follistatin-like 1; FSTL1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ile308

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human FSTL1 (NP_009016.1) precursor (Met 1-Ile 308) with a carboxy-terminal polyhistidine tag was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for FSTL1 recombinant protein

Background: Follistatin-related protein 1 (FSTL1) is an extracellular glycoprotein whose functional significance in physiological and pathological processes is incompletely understood. Recently, we have shown that FSTL1 acts as a muscle-derived secreted factor that is up-regulated by Akt activation and ischemic stress and that FSTL1 exerts favorable actions on the heart and vasculature. Here, we sought to identify the receptor that mediates the cellular actions of FSTL1. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. FSTL1 is thought to be an autoantigen associated with rheumatoid arthritis. DIP2A functions as a novel receptor that mediates the cardiovascular protective effects of FSTL1. Experiment results have provided in vivo and in vitro evidence to demonstrate that Fstl1 modulates lung development and alveolar maturation, in part, through BMP4 signaling.

References

Rosenberg MI, et al. (2006) MyoD inhibits Fstl1 and Utrn expression by inducing transcription of miR-206. J Cell Biol. 175(1): 77-85.Ouchi N, et al. (2010) DIP2A functions as a FSTL1 receptor. J Biol Chem. 285(10): 7127-34.Geng Y, et al. (2011) Follistatin-like 1 (Fstl1) is a bone morphogenetic protein (BMP) 4 signaling antagonist in controlling mouse lung development. Proc Natl Acad Sci U S A. 108(17): 7058-63.

NCBI and Uniprot Product Information

• NCBI GI #: 5901956
• NCBI GeneID: 11167
• NCBI Accession #: NP_009016.1
• NCBI GenBank Nucleotide #: NM_007085.4
• UniProt Accession #: Q12841; Q549Z0; A8K523; B4DTT5; D3DN90
• Molecular Weight: 31,228 Da
• NCBI Official Full Name: follistatin-related protein 1
• NCBI Official Synonym Full Names: follistatin-like 1
• NCBI Official Symbol: FSTL1
• NCBI Official Synonym Symbols: FRP; FSL1; MIR198
• NCBI Protein Information: follistatin-related protein 1; follistatin-like protein 1
• UniProt Protein Name: Follistatin-related protein 1
• Protein Family: Follistatin-related protein
• UniProt Gene Name: FSTL1
• UniProt Synonym Gene Names: FRP
• UniProt Entry Name: FSTL1_HUMAN

NCBI Description

This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. [provided by RefSeq, Jul 2008]

Uniprot Description

FSTL1: May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q13.33

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; protein binding; calcium ion binding

Biological Process: BMP signaling pathway; response to starvation