BTN3A3 recombinant protein

BTN3A3 Protein, Human, Recombinant (mFc Tag)

Cat. Num. AAA8121407

• Gene Names: BTN3A3; BTF3
• Purity: >95% as determined by SDS-PAGE
• Synonyms: BTN3A3; BTN3A3 Protein; Human; Recombinant (mFc Tag); Human BTN3A3 Protein (Fc Tag); BTF3 Protein; BTN3.3 Protein; butyrophilin subfamily 3 member A3; BTN3A3 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Trp248

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 30
• Tag: C-mouse IgG1-Fc
• Protein Construction: A DNA sequence encoding the human BTN3A3 (O00478-1)(Met1-Trp248) was fused with Fc region of mouse IgG at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for BTN3A3 recombinant protein

Background: The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. The butyrophilin 3 (BTN3) receptors are implicated in the T lymphocytes regulation and present a wide plasticity in mammals. A thorough phylogenetic analysis reveals a concerted evolution of BTN3 characterized by a strong and recurrent homogenization of the region encoding the signal peptide and the immunoglobulin variable (IgV) domain in Hominoids, where the sequences of BTN3A1 or BTN3A3 are replaced by BTN3A2 sequence.

NCBI and Uniprot Product Information

• NCBI GI #: 338797729
• NCBI GeneID: 10384
• NCBI Accession #: NP_001229732.1
• NCBI GenBank Nucleotide #: NM_001242803.1
• UniProt Accession #: O00478; B4DWI7; E9PCP5
• Molecular Weight: 65,002 Da
• NCBI Official Full Name: butyrophilin subfamily 3 member A3 isoform c
• NCBI Official Synonym Full Names: butyrophilin, subfamily 3, member A3
• NCBI Official Symbol: BTN3A3
• NCBI Official Synonym Symbols: BTF3
• NCBI Protein Information: butyrophilin subfamily 3 member A3; butyrophilin 3
• UniProt Protein Name: Butyrophilin subfamily 3 member A3
• Protein Family: Butyrophilin subfamily
• UniProt Gene Name: BTN3A3
• UniProt Synonym Gene Names: BTF3
• UniProt Entry Name: BT3A3_HUMAN

NCBI Description

The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1; MIM 613590) and BTN3 (e.g., BNT3A3) genes, which have undergone tandem duplication, resulting in 3 copies of each (summary by Smith et al., 2010 [PubMed 20208008]).[supplied by OMIM, Nov 2010]

Uniprot Description

Function: Plays a role in T-cell responses in the adaptive immune response. Ref.10

Subunit structure: Homodimer. Ref.11

Subcellular location: Cell membrane; Single-pass type I membrane protein Ref.9 Ref.10.

Tissue specificity: Detected in peripheral blood mononuclear cells and in T-cells (at protein level). Detected in spleen and lymphocytes. Ref.8

Post-translational modification: N-glycosylated. Ref.8

Sequence similarities: Belongs to the immunoglobulin superfamily. BTN/MOG family.Contains 1 B30.2/SPRY domain.Contains 2 Ig-like V-type (immunoglobulin-like) domains.