Human cytokine-like 1 ELISA Kit | CYTL1 elisa kit

Human Cytokine-like protein 1, CYTL1 ELISA Kit

Cat. Num. AAA910123

• Gene Names: CYTL1; C17; C4orf4
• Reactivity: Human
• Synonyms: cytokine-like 1; Human Cytokine-like protein 1; CYTL1 ELISA Kit; Human Cytokine-like protein 1 (CYTL1) ELISA kit; C17; C4orf4; cytokine-like protein C17; CYTL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CYTL1. No significant cross-reactivity or interference between human CYTL1 and analogues was observed.
• Sequence Length: 136

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.156 ng/ml -10 ng/ml.
• Sensitivity: The minimum detectable dose of human CYTL1 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYTL1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYTL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYTL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYTL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYTL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 8922108
• NCBI GeneID: 54360
• NCBI Accession #: NP_061129.1
• NCBI GenBank Nucleotide #: NM_018659.2
• UniProt Accession #: Q9NRR1
• Molecular Weight: 15,577 Da
• NCBI Official Full Name: cytokine-like protein 1
• NCBI Official Synonym Full Names: cytokine-like 1
• NCBI Official Symbol: CYTL1
• NCBI Official Synonym Symbols: C17; C4orf4
• NCBI Protein Information: cytokine-like protein 1; cytokine-like protein C17
• UniProt Protein Name: Cytokine-like protein 1
• Protein Family: Cytokine-like protein
• UniProt Gene Name: CYTL1
• UniProt Synonym Gene Names: C4orf4; UNQ1942/PRO4425
• UniProt Entry Name: CYTL1_HUMAN

NCBI Description

C17 is a cytokine-like protein specifically expressed in bone marrow and cord blood mononuclear cells that bear the CD34 (MIM 142230) surface marker (Liu et al., 2000 [PubMed 10857752]).[supplied by OMIM, Mar 2008]

Uniprot Description

CYTL1:

Protein type: Secreted; Secreted, signal peptide; Transcription factor; Cell development/differentiation

Chromosomal Location of Human Ortholog: 4p16-p15

Cellular Component: extracellular space

Molecular Function: receptor binding

Biological Process: chondroitin sulfate proteoglycan biosynthetic process; chondrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; signal transduction; inner ear development