GPHA2 recombinant protein

GPHA2 Protein, Human, Recombinant (mFc Tag)

Cat. Num. AAA8121564

• Gene Names: GPHA2; A2; GPA2; ZSIG51
• Purity: >85% as determined by SDS-PAGE
• Synonyms: GPHA2; GPHA2 Protein; Human; Recombinant (mFc Tag); Human GPHA2/Glycoprotein hormone alpha 2 Protein (Fc Tag); A2 Protein; GPA2 Protein; ZSIG51 Protein; glycoprotein hormone alpha 2; GPHA2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Tyr129

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 24
• Tag: C-mouse IgG1-Fc
• Protein Construction: A DNA sequence encoding the human GPHA2 (Q96T91)(Met1-Tyr129) was fused with Fc region of mouse IgG at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GPHA2 recombinant protein

Background: GPHA2 is a member of the glycoprotein hormones subunit alpha family. Glycoprotein hormones consist of two subunits, the common alpha- and specific beta-subunits, which associate noncovalently to form a heterodimer. The alpha-subunit combines with four distinct beta-subunits giving rise to four biologically active hormones in the human: FSH, LH, TSH, and CG. GPHA2 and glycoprotein hormone beta 5 (GPHB5) can form a noncovalent heterodimer. GPHA2 can be detected in a variety of tissues. Recombinant A2/B5 heterodimeric glycoproteins activate human TSH receptors, but not LH and FSH receptors, and shows high affinity to TSH receptors in a radioligand receptor assay. The heterodimer also stimulates cAMP production and thymidine incorporation by cultured thyroid cells and increases serum thyroxine levels in TSH-suppressed rats in vivo. This new heterodimeric glycoprotein hormone was named Thyrostimulin based on its thyroid-stimulating activity. The expression of Thyrostimulin in the anterior pituitary known to express TSH receptors suggested a paracrine mechanism.

References

Hsu SY. et al., 2002, Science. 295 (5555): 671-4. Suzuki C. et al., 2007, Regul Pept. 142 (1-2): 60-7. Breous E. et al., 2006, Mol Cell Endocrinol. 245 (1-2): 169-80.

NCBI and Uniprot Product Information

• NCBI GI #: 18640742
• NCBI GeneID: 170589
• NCBI Accession #: NP_570125.1
• NCBI GenBank Nucleotide #: NM_130769.3
• UniProt Accession #: Q96T91; Q52LE2
• Molecular Weight: 14,163 Da
• NCBI Official Full Name: glycoprotein hormone alpha-2
• NCBI Official Synonym Full Names: glycoprotein hormone alpha 2
• NCBI Official Symbol: GPHA2
• NCBI Official Synonym Symbols: A2; GPA2; ZSIG51
• NCBI Protein Information: glycoprotein hormone alpha-2; cysteine knot protein; glycoprotein alpha 2; putative secreted protein Zsig51; thyrostimulin subunit alpha
• UniProt Protein Name: Glycoprotein hormone alpha-2
• Protein Family: Glycoprotein hormone
• UniProt Gene Name: GPHA2
• UniProt Synonym Gene Names: GPA2; ZSIG51
• UniProt Entry Name: GPHA2_HUMAN

NCBI Description

GPHA2 is a cystine knot-forming polypeptide and a subunit of the dimeric glycoprotein hormone family (Hsu et al., 2002 [PubMed 12089349]).[supplied by OMIM, Mar 2008]

Uniprot Description

GPHA2: Stimulates the thyroid. Binds and activates THSR, leads to increased cAMP production. Belongs to the glycoprotein hormones subunit alpha family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: extracellular region

Molecular Function: hormone activity