Cathepsin K (CTSK) Recombinant Protein | CTSK recombinant protein

Recombinant Cathepsin K (CTSK)

Cat. Num. AAA2009972

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cathepsin K (CTSK); Recombinant Cathepsin K (CTSK); CTSK recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-PDSVD YRKKGYVTPV KNQGQCGSCW AFSSVGALEG QLKKKTGKLL NLSPQNLVDC VSENDGCGGG YMTNAFQYVQ KNRGIDSEDA YPYVGQEESC MYNPTGKAAK CRGYREIPEG NEKALKRAVA RVGPVSVAID ASLTSFQFYS KGVYYDESCN SDNLNHAVLA VGYGIQKGNK HWIIKNSWGE NWGNKGYILM ARNKNNACGI ANLASFPKM
• Sequence Length: 329

• Applicable Applications for CTSK recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rhesus monkey (Simian)
• Expression System: Prokaryotic expression
• Residues: Pro116~Met329 (Accession # P61277) with N-terminal His-Tag
• Subcellular Location: Lysosome
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 24.9kDa
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 74136185
• NCBI GeneID: 574112
• NCBI Accession #: NP_001027984.1
• NCBI GenBank Nucleotide #: NM_001032812.2
• UniProt Accession #: P61277; O77641
• Molecular Weight: 24.9kDa
• NCBI Official Full Name: cathepsin K
• NCBI Official Symbol: CTSK
• NCBI Protein Information: cathepsin K; cathepsin K preproprotein
• UniProt Protein Name: Cathepsin K
• Protein Family: Cathepsin
• UniProt Gene Name: CTSK
• UniProt Entry Name: CATK_MACMU

Uniprot Description

Function: Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation

By similarity.

Catalytic activity: Broad proteolytic activity. With small-molecule substrates and inhibitors, the major determinant of specificity is P2, which is preferably Leu, Met > Phe, and not Arg.

Subcellular location: Lysosome

By similarity.

Sequence similarities: Belongs to the peptidase C1 family.