Rabbit anti-Human Nak1 Polyclonal Antibody | anti-NR4A1 antibody

Nak1 (Nur77)

Cat. Num. AAA611939

• Gene Names: NR4A1; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Nak1; Polyclonal Antibody; Nak1 (Nur77); Anti -Nak1 (Nur77); anti-NR4A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes Human Nuclear Receptor Nak1 (NR4A1). Species Crossreactivity: Human.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% sodium azide.

• Applicable Applications for anti-NR4A1 antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry.; Dilution: Immunohistochemistry: 15-20ug/ml
• Immunogen: Synthetic peptide, KLH-conjugated. Epitope: internal domain of human
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(NR4A1 / NUR77 (21ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human primary follicles, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-NR4A1 antibody

Nak1 is a member of the steroid/thyroid hormone receptor superfamily. The gene for Nak-1 is induced rapidly by androgens/growth factors and may have functions related to cell proliferation, differentiation and apoptosis (1). Liu et al (2) have demonstrated that mouse nur77 is necessary for induced apoptosis in T-cell hybridomas and can also be induced during early mitogenesis. Nak1 is a phosphoprotein and its size ranges from 67kD to 88kD, depending on post-translational modifications.

Product Categories/Family for anti-NR4A1 antibody

Antibodies; Abs to Hormones; Steroids

NCBI and Uniprot Product Information

• NCBI GI #: 127819
• NCBI GeneID: 3164
• UniProt Accession #: P22736; Q15627; B4DML7
• Molecular Weight: 64,463 Da
• NCBI Official Full Name: Nuclear receptor subfamily 4 group A member 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 4, group A, member 1
• NCBI Official Symbol: NR4A1
• NCBI Official Synonym Symbols: HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
• NCBI Protein Information: nuclear receptor subfamily 4 group A member 1; ST-59; hormone receptor; TR3 orphan receptor; steroid receptor TR3; testicular receptor 3; early response protein NAK1; orphan nuclear receptor HMR; orphan nuclear receptor TR3; nuclear hormone receptor NUR/77; growth factor-inducible nuclear protein N10; nerve growth factor IB nuclear receptor variant 1
• UniProt Protein Name: Nuclear receptor subfamily 4 group A member 1
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR4A1
• UniProt Synonym Gene Names: GFRP1; HMR; NAK1; Nur77
• UniProt Entry Name: NR4A1_HUMAN

NCBI Description

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Nur77: an orphan nuclear receptor and immediate-early gene that regulates cellular proliferation, apoptosis, inflammation, and glucose metabolism. Induced by exercise in muscle and is a functional regulator of glucose metabolism in skeletal muscle. Its level decreases in the muscle of obese insulin-resistant men. Acts concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. A mediator of TCR-directed thymocyte apoptosis. TCR-signaling induces a FAIM/Akt/Nur77 signaling pathway that is critical for modulating apoptosis in developing thymocytes. A physiological substrate of the MEK-ERK-RSK cascade that modulates nuclear export and intracellular translocation during T cell death. Binds DNA as a monomer. Interacts with GADD45GIP1. Overexpression of Nur77 induces the expression of both p300 and HDAC1. Acetylation by p300 and HDAC1 may regulate the rapid turnover of Nur77 protein.

Protein type: Apoptosis; DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity

Biological Process: epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; fibroblast growth factor receptor signaling pathway; intracellular receptor-mediated signaling pathway; phosphoinositide-mediated signaling; cell migration during sprouting angiogenesis; nerve growth factor receptor signaling pathway; innate immune response; gene expression; positive regulation of endothelial cell proliferation; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; signal transduction