Cathepsin S (CTSS) Recombinant Protein | CTSS recombinant protein

Recombinant Cathepsin S (CTSS)

Cat. Num. AAA2011362

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cathepsin S (CTSS); Recombinant Cathepsin S (CTSS); CTSS recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag Form & Buffer: Supplied as solution form in PBS, pH 74, con, its sequence is listed below.; MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-LPDTVDWR EKGCVTEVKY QGSCGACWAF SAVGALEGQL KLKTGKLISL SAQNLVDCSN EEKYGNKGCG GGYMTEAFQY IIDNGGIEAD ASYPYKATDE KCHYNSKNRA ATCSRYIQLP FGDEDALKEA VATKGPVSVG IDASHSSFFF YKSGVYDDPS CTGNVNHGVL VVGYGTLDGK DYWLVKNSWG LNFGDQGYIR MARNNKNHCG IASYCSYPE
• Sequence Length: 340

• Applicable Applications for CTSS recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Leu123~Glu339 (Accession # O70370) with two N-terminal Tags, His-tag and S-tag
• Form & Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.
• Predicted isoelectric point: 5.6
• Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 510025483
• NCBI GeneID: 13040
• NCBI Accession #: NP_067256.4
• NCBI GenBank Nucleotide #: NM_021281.3
• UniProt Accession #: O70370; O54973; E9QK18
• Molecular Weight: 29.3kDa
• NCBI Official Full Name: cathepsin S isoform 2 preproprotein
• NCBI Official Synonym Full Names: cathepsin S
• NCBI Official Symbol: Ctss
• NCBI Protein Information: cathepsin S; Cat S
• UniProt Protein Name: Cathepsin S
• Protein Family: Cathepsin
• UniProt Gene Name: Ctss
• UniProt Synonym Gene Names: Cats
• UniProt Entry Name: CATS_MOUSE

NCBI Description

This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. Alternative splicing results in multiple transcript variants, which encode preproproteins that are proteolytically processed to generate mature protein products. This enzyme is secreted by antigen-presenting cells during inflammation and may induce pain and itch via activation of G-protein coupled receptors. Homozygous knockout mice for this gene exhibit impaired wound healing, reduced tumorigenesis in a pancreatic cancer model, and reduced pathogenesis in a myasthenia gravis model. [provided by RefSeq, Aug 2015]

Uniprot Description

CTSS: Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond- specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N. Belongs to the peptidase C1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.22.27

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; membrane; lysosome

Molecular Function: collagen binding; peptidase activity; proteoglycan binding; hydrolase activity; cysteine-type endopeptidase activity; fibronectin binding; laminin binding; cysteine-type peptidase activity

Biological Process: collagen catabolic process; proteolysis involved in cellular protein catabolic process; regulation of sensory perception of pain; proteolysis; bone resorption; positive regulation of inflammatory response