Cathepsin K (CTSK) Recombinant Protein | CTSK recombinant protein

Recombinant Cathepsin K (CTSK)

Cat. Num. AAA2012295

• Gene Names: Ctsk; catK; Ms10q; MMS10-Q; AI323530
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cathepsin K (CTSK); Recombinant Cathepsin K (CTSK); CTSK recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- GRVPDSID YRKKGYVTPV KNQGQCGSCW AFSSAGALEG QLKKKTGKLL ALSPQNLVDC VTENYGCGGG YMTTAFQYVQ QNGGIDSEDA YPYVGQDESC MYNATAKAAK CRGYREIPVG NEKALKRAVA RVGPISVSID ASLASFQFYS RGVYYDENCD RDNVNHAVLV VGYGTQKGSK HWIIKNSWGESWGNKGYALL ARNKNN
• Sequence Length: 329

• Applicable Applications for CTSK recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gly113~Asn316 (Accession # P55097) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Lysosome
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 7.6
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 31982433
• NCBI GeneID: 13038
• NCBI Accession #: NP_031828.2
• NCBI GenBank Nucleotide #: NM_007802.4
• UniProt Accession #: P55097; O88718
• Molecular Weight: 54.3kDa
• NCBI Official Full Name: cathepsin K
• NCBI Official Synonym Full Names: cathepsin K
• NCBI Official Symbol: Ctsk
• NCBI Official Synonym Symbols: catK; Ms10q; MMS10-Q; AI323530
• NCBI Protein Information: cathepsin K; Cat K; minisatellite 10q detected by probe MMS10
• UniProt Protein Name: Cathepsin K
• Protein Family: Cathepsin
• UniProt Gene Name: Ctsk
• UniProt Entry Name: CATK_MOUSE

Uniprot Description

CTSK: Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Defects in CTSK are the cause of pycnodysostosis (PKND). PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.38; Protease

Cellular Component: extracellular space; lysosome; cytoplasm

Molecular Function: collagen binding; peptidase activity; proteoglycan binding; hydrolase activity; fibronectin binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: collagen catabolic process; proteolysis involved in cellular protein catabolic process; proteolysis; bone resorption