Cathepsin L (CTSL) Recombinant Protein | CTSL recombinant protein

Recombinant Cathepsin L (CTSL)

Cat. Num. AAA2009935

• Gene Names: Ctsl1; CatL; Ctsl; CATHL
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cathepsin L (CTSL); Recombinant Cathepsin L (CTSL); CTSL recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-IPKTVDW REKGCVTPVK NQGQCGSCWA FSASGCLEGQ MFLKTGKLIS LSEQNLVDCS HDQGNQGCNG GLMDFAFQYI KENGGLDSEE SYPYEAKDGS CKYRAEYAVA NDTGFVDIPQ QEKALMKAVA TVGPISVAMD ASHPSLQFYS SGIYYEPNCS SKDLDHGVLV VGYGYEGT
• Sequence Length: 334

• Applicable Applications for CTSL recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Ile114~Thr288 (Accession # P07154) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6978723
• NCBI GeneID: 25697
• NCBI Accession #: NP_037288.1
• NCBI GenBank Nucleotide #: NM_013156.2
• UniProt Accession #: P07154; Q9QV07
• Molecular Weight: 20.5kDa
• NCBI Official Full Name: cathepsin L1 preproprotein
• NCBI Official Synonym Full Names: cathepsin L1
• NCBI Official Symbol: Ctsl1
• NCBI Official Synonym Symbols: CatL; Ctsl; CATHL
• NCBI Protein Information: cathepsin L1; MEP; CP-2; cyclic protein 2; major excreted protein
• UniProt Protein Name: Cathepsin L1
• UniProt Gene Name: Ctsl
• UniProt Synonym Gene Names: Ctsl1; CP-2; MEP
• UniProt Entry Name: CATL1_RAT

NCBI Description

mouse homolog is deleted in the CD4 T-cell-deficient mouse mutant nacht (nkt); may play a role in CD4+ T-cell selection in the thymus [RGD, Feb 2006]

Uniprot Description

CTSL2: Cysteine protease. May have an important role in corneal physiology. Belongs to the peptidase C1 family.

Protein type: Secreted; Secreted, signal peptide; EC 3.4.22.15; Protease

Cellular Component: extracellular space; neuron projection; microvillus; lysosome; perikaryon; secretory granule; apical part of cell; cytoplasm; nucleolus; vacuole; cytoplasmic vesicle; nucleus; external side of plasma membrane

Molecular Function: collagen binding; proteoglycan binding; histone binding; cysteine-type endopeptidase activity; fibronectin binding; protein complex binding; aminopeptidase activity; cysteine-type carboxypeptidase activity; kininogen binding; peptide binding; cysteine-type peptidase activity

Biological Process: adaptive immune response; hair follicle morphogenesis; response to glucocorticoid stimulus; male gonad development; Sertoli cell differentiation; multicellular organismal aging; decidualization; proteolysis; cellular response to starvation; response to organic cyclic substance; collagen catabolic process; protein autoprocessing; proteolysis involved in cellular protein catabolic process; response to glucose stimulus; autophagic cell death; nerve development; spermatogenesis; cell communication; protein processing