Cystatin SA ELISA Pair Set | CST2 elisa pair set

Cystatin SA Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118382

• Synonyms: Cystatin SA; Cystatin SA Matched ELISA Antibody Pair Set; Human; Human Cystatin SA/CST2 ELISA Pair Set; cystatin SA; CST2 Matched ELISA Antibody Pair Set; MGC71924 Matched ELISA Antibody Pair Set; CST2 elisa pair set

• Specificity: Quantitative determination of Human Cystatin SA

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 4.69-300pg/mL
• Sensitivity: 4.7pg/ml
• Materials Provided: 1. Capture Antibody 1.0 mg/mL of rabbit anti-CST2 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in CBS before coating. ; 2. Detection Antibody 0.25 mg/mL rabbit anti-CST2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 15 ng of recombinant CST2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.3ng/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for CST2 elisa pair set

Cystatin-SA, also known as Cystatin-2, Cystatin-S5 and CST2, is a secreted protein which belongs to thecystatin family. Cystatin-2/CST2 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). It is also expressed in submandibular gland and parotid gland. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The CST1, CST2, CST4, and CST5 are expressed in differential, tissue-specific patterns. Expression of CST2 and CST5 is restricted to the submandibular and parotid glands, while CST1 and CST4 are expressed in these tissues and in the lacrimal gland.

This Cystatin SA Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Cystatin SA. It contains Human Cystatin SA capture antibody, Human Cystatin SA detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Cystatin SA protein.

References

Saitoh E., et al., (1987), Human cysteine-proteinase inhibitors: nucleotide sequence analysis of three members of the cystatin gene family. Gene 61:329-338.Zhang Z., et al., (2004), Signal peptide prediction based on analysis of experimentally verified cleavage sites.Protein Sci. 13:2819-2824.Deloukas P., et al., (2001), The DNA sequence and comparative analysis of human chromosome 20.Nature 414:865-871.