Osteomodulin ELISA Pair Set | OMD elisa pair set

Osteomodulin Matched ELISA Antibody Pair Set, Mouse

Cat. Num. AAA8118503

• Synonyms: Osteomodulin; Osteomodulin Matched ELISA Antibody Pair Set; Mouse; Mouse Osteomodulin/OMD ELISA Pair Set; osteomodulin; OSAD Matched ELISA Antibody Pair Set; SLRR2C Matched ELISA Antibody Pair Set; OMD elisa pair set

• Specificity: Quantitative determination of Mouse Osteomodulin

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 12.5-800pg/mL
• Sensitivity: 12.5pg/ml
• Materials Provided: 1. Capture Antibody 1.0 mg/mL of rabbit anti-mouse OMD monoclonal antibody. Dilute to a working concentration of 2ug/mL in CBS before coating. ; 2. Detection Antibody 0.5 mg/mL rabbit anti-mouse OMD monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 232 ng of recombinant mouse OMD. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.8ng/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for OMD elisa pair set

Osteomodulin (OMD), also known as Osteoadherin (OSAD), Keratan sulfate proteoglycan osteomodulin, KSPG osteomodulin, and SLRR2C, is a secreted protein that belongs to the small leucine-rich proteoglycan (SLRP) family and Class II subfamily. SLRP family proteins are normally found in extracellular matrices, but Osteomodulin is the only member restricted to mineralized tissues. Osteomodulin is primarily expressed by osteoblasts and might have a role in the regulation of mineralization. In bone, OSAD has been localized in the primary spongiosa within the bovine fetal rib growth plate. Moreover, in situ hybridization has shown expression of OSAD in osteoblasts close to the cartilage and bone border in the growth plate of rat femur. OSAD may play an important role during tooth development and biomineralization of dentin. Osteomodulin is a cell binding keratan sulfate proteoglycan that was recently isolated from mineralized bovine bone and subsequently cloned and sequenced. Osteomodulin may be implicated in biomineralization processes. It has a function in the binding of osteoblasts via the alpha (V) beta (3)-integrin. Osteomodulin is likely an osteoblast maturation marker that is induced by osteoclast activity. Osteomodulin is also an early marker for terminally differentiated matrix producing osteoblasts.

This Osteomodulin Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse Osteomodulin. It contains Mouse Osteomodulin capture antibody, Mouse Osteomodulin detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse Osteomodulin protein.

References

Buchaille R, et al. (2000) Expression of the small leucine-rich proteoglycan osteoadherin/osteomodulin in human dental pulp and developing rat teeth. Bone. 27(2): 265-70.Petersson U, et al. (2003) Identification, distribution and expression of osteoadherin during tooth formation. Eur J Oral Sci. 111(2): 128-36.Rehn AP, et al. (2006) Differential regulation of osteoadherin (OSAD) by TGF-beta1 and BMP-2. Biochem Biophys Res Commun. 349(3): 1057-64.